畜禽体外培养细胞生长规律研究  

An Research of the Growth Rule of Cells Cultured in Vitro of Livestock and Poultry

作　　者：陆涛峰[1] 刘长青[1,2] 吴宏梅[1] 张艳艳[1,3] 刘帅[1,4] 包阿东[1,4] 张文秀[1] 关伟军[1] 马月辉[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]中国海洋大学生命科学与技术学部,青岛266003 [3]西北农林科技大学,杨凌712100 [4]内蒙古农业大学,呼和浩特010000

出　　处：《中国畜牧兽医》2008年第11期65-69,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家“863”计划项目(2006AA10Z198);国家“863”计划项目(2007AA10Z170)

摘　　要：本研究利用组织块贴壁培养法对鲁西黄牛、蒙古马、北京鸭和狼山鸡等4个品种体外培养细胞的生长、分化规律进行了研究。通过绘制其生长曲线和在激光共聚焦显微镜下拍摄细胞生长过程,初步分析成纤维细胞的生长、分化规律。试验结果表明,4个品种的细胞生长曲线均呈明显的"S"形,家畜和家禽的细胞潜伏期和停滞期的长短以及细胞群体倍增时间均有明显差异,蒙古马和狼山鸡体外培养细胞的单个细胞分裂期也有显著差异,蒙古马为97 min,而狼山鸡为85 min。本试验构建的4个品种成纤维细胞系不仅使畜禽遗传资源在细胞水平永久保存下来,而且为分子生物学研究提供宝贵材料。In order to study on the growth and differentiation rule of cells cultured in vitro of Luxi cattle, Mongolia horse, Peking duck and Langshan chicken,the vitro four cell lines were established successfully in this study with tissure attachment culture method. Analysis on the growth and differentiation rule of fibroblast cell through rendering the growth curve and photographing the cells growth process by Confocal Laser Scanning Microscopy. The experimental results indicate that., the four cell growth curves are all showed significantly s-shape, the length of retention period and incubation period are obviously different between livestock and poultry cells , and the doubling time for livestock and poultry cells are also, in addition, the single cell division period of Mongolia horse and Langshan chicken cells cultured in vitro are obviously different too, the former is 97 min and the latter is 85 min.

关 键 词：畜禽 成纤维细胞 生长规律 生长曲线 分裂期 

分 类 号：S813.3[农业科学—畜牧学]