应用毛细管区段灌注技术对胶束电动毛细管柱色谱法分离6种二硝基甲苯异构体条件的优化  

Application of CPF Technique to the Optimization of Conditions for Separation of 6 Isomers of Dinitrotoluene by MECC

作　　者：高苏亚[1,2] 党高潮[2] 李华[3] 

机构地区：[1]西安医学院药学系,西安710021 [2]西北大学化学系 [3]西北大学分析科学研究所,西安710069

出　　处：《理化检验（化学分册）》2008年第11期1024-1027,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金资助项目(20675063)

摘　　要：用区段灌注技术及胶束电动毛细管柱色谱法并应用正交设计法研究及优化了6种二硝基甲苯异构体的分离条件。采用在30 mmol·L^(-1)SDS,10 mmol·L^(-1)Brij-35,6 mmol·L^(-1)β- CD,20 mmol·L^(-1)硼砂及5 mmol·L^(-1)磷酸二氢钠溶液组成的缓冲溶液中对6种二硝基甲苯异构体进行分离,进样时间6 s,分离电压18 kV,分离效果好,基线平稳,背景小,检测灵敏度高。Separation of 6 isomers of dinitrotoluene （DNT） was studied by the capillary partial filling （CPF） micellar electrokinetic capillary column chromatography （MECC） and conditions for the separation were optimized by using the method of orthogonal experimental design. A buffer mixture containing solutions of 30 mmol · L^-1 SDS, 10 mmol · L^-1 Brij 35, 6 mmol · L^-1 β-CD, 20 mmol · L^-1 borax and 5 mmol · L^-1 NaH2PO4 was used in the separation, and time of sample introduction of 6 s, as well as the separation voltage of 18 kV were adopted for the separation. Under the optimized conditions, stable and flat base line, low background, highly sensitive detection and high separation efficiency were achieved.

关 键 词：区段灌注 胶束电动毛细管色谱 畀构体分离 二硝基甲苯 

分 类 号：O657.3[理学—分析化学]