PCR结合变性高效液相色谱快速检测水产品中河流弧菌  

Detection of Vibrio fluvialis in food by PCR-DHPLC

作　　者：曹际娟[1] 赵昕[1] 孙哲平[1] 于珂[1] 徐君怡[1] 郑秋月[1,2] 

机构地区：[1]辽宁出入境检验检疫局,辽宁大连116001 [2]沈阳农业大学,沈阳110161

出　　处：《中国卫生检验杂志》2008年第11期2187-2189,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家"十一五"科技支撑计划课题(2006BAK02A13)

摘　　要：目的:应用PCR结合变性高效液相色谱技术实现水产品中河流弧菌的快速鉴定。方法:利用河流弧菌的toxR基因序列设计引物,并对该方法进行特异性、精密度和实际应用等方面的考察。结果:用该方法未检测到河流弧菌的近源种及其他细菌的阳性吸收峰,表现出了很好的特异性。结论:用PCR结合变性高效液相色谱检测河流弧菌,不仅特异性好、灵敏度高,且方法简便、快速。Ojbective： To use PCR and denaturing high -performance liquid chromatography to identify Vibrio fluvialis rapidly. Methods：Primers for specific PCR amplification were designed based on the transmembrane regulatory protein ToxR gene of Vibrio fluvialis. Meanwhile,its specificity and stability were examined. Results：Using this method to detect Vibrio fluvialis and other Bacteriaes, only Vibrio fluvialis can be detected. Conclusion ： A PCR - DHPLC assay amplification targeting at the ToxR gene of Vibrio fluvialis is a rapid, specific, sensitive and relatively simple method for the identification of Vibrio fluvialis.

关 键 词：PCR—DHPLC 河流弧菌 toxR基因 检测 

分 类 号：O657.72[理学—分析化学]