固相萃取结合高效液相色谱测定鸡肉中10种磺胺类药物残留  

Simultaneous determination of sulfonamide residues in chicken muscle by solid phase extraction and high performance liquid chromatography

作　　者：吴平谷[1] 冯靓[1] 王强[2] 赵永信[1] 谭莹[1] 张晶[1] 汤鋆[1] 应英[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310009 [2]浙江省农业科学院,杭州310020

出　　处：《中国卫生检验杂志》2008年第11期2201-2203,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省重点科研农业资助项目(2005C22029);浙江省重大科技攻关资助项目(2005C12007)

摘　　要：目的：建立鸡肉中10种磺胺类药物残留的HPLC测定方法。方法：鸡肉经过甲醇提取，正已烷脱脂，SLW固相萃取柱净化，用HPLC法测定鸡肉中10种磺胺类药物的含量。结果：当鸡肉样品添加10种磺胺类药物为0．05～0．2mg／kg时，方法回收率为67．4％～95．6％，变异系数为4．8％～11．5％，10种磺胺类药物最低检出浓度为0．01mg／g。结论：以HPLC法测定鸡肉中10种磺胺类药物残留，操作简便、定量准确可靠。Objective：To establish an HPLC method for qnantization of 10 sulfonamides residues in chicken muscle. Methods： Chicken muscle was extracted with methanol, defatted with n - hexane and cleaned up with SLW solid - phase extraction. Then the concentration of 10 sulfonamides residues was determined by HPLC. Results：The recoveries of 10 sulfonamides residues in chicken muscle were 67.4% - 95.6%, with relative standard deviations between 4. 8% and 11.5%. While the contents of added standard were 0. 05 - 0. 2 mg/kg, and the minimum detected limits of this method were 0. 01 mg/kg. Conclusion：This is a sim- ple, accurate and reliable method for determination of 10 sulfonamides residues in chicken muscle.

关 键 词：固相萃取 高效液相色谱 测定 磺胺 残留 

分 类 号：O657.72[理学—分析化学]