公共场所从业人员无症状HBV携带者中检测前S1抗原的必要性探讨  

Neccesity of detecting Pre-S1 antigen in asymptomatic carriers of public practitioners

作　　者：李兰芳[1] 肖春燕 魏华江[3] 黄明元[1] 潘翊[1] 

机构地区：[1]广州市海珠区疾病预防控制中心,广州510288 [2]广州市天河区妇幼保健院,广州510620 [3]华南师范大学激光生命科学研究所,广州510631

出　　处：《中国卫生检验杂志》2008年第11期2327-2329,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：探讨公共场所从业人员无症状HBV携带者中检测前S1抗原（Pre—S1Ag）的必要性。方法：Pre—S1Ag、HBV血清学标志物（HBV—M）采用ELISA法，HBV DNA检测采用实时荧光定量PCR法。结果：2006．06．05—2007．06．05公共场所体检人数10034人，单阳（单一HBsAg阳性）人数532人（5．30％），双阳（HBsAg、HBeAg阳性）人数231人（2．30％），单阳与双阳阳性检出率比较,χ^2=123．44，P〈0．05，两者差别有统计学意义。单阳阳性率显著高于双阳阳性率。HBV—DNA阴阳性体检者血清标本中，HBV—DNA与Pre—S1Ag比较：χ^2=1．13，P〉0．05，两者差别无统计学意义。HBeAg阳性组中，Pre—S1Ag、HBV—DNA的检查率分别为：97．0％、93．9％，三者之间有较高符合率；HBV—DNA与Pre—S1Ag比较：χ^2=2．44，P〉0．05。HBeAg阴性组中，HBV—DNA与Pre—S1Ag比较：χ^2=0．46，P〉0．05。两组差异均无统计学意义。将HBeAg阳性组与阴性组的Pre—S1Ag阳性率进行比较：χ^2=343．73，P〈0．05，两者差别有统计学意义。结论：作为反映乙肝病毒复制和传染性的血清学标志，前S1抗原和DNA与HBeAg密切相关，且更敏感，对“乙肝五项”检测有重要的补充和加强作用。前S1抗原具有独立的检测价值。Objective：To determine the necessity of detecting Pre - S1 antigen （ Pre - S1Ag） in asymptomatic carriers of public practitioners. Methods：Pre -SlAg and HBV markers （HBV -M） of all samples were detected by ELISA, HBV DNA of all samples was detected by fluorescent - quantitativing PCR method. Results： In the 10034 blood samples from 2006. 06.05 to 2007. 06. 05 collected by physical examinations, the positive cases of HBsAg were 532 （5. 30% ）, and the positive cases of both HBsAg and HBeAg were 231 （2. 30% ）. There was significant difference between these two groups （χ^2 = 123.44,P 〈0. 05）. No significant difference was observed between the HBV- DNA and Pre- SlAg （χ^2 = 1.13 ,P 〉0. 05 ） in the HBV -DNA negative and positive groups. In the HBeAg positive group, the positive rates ofPre - SlAg and HBV - DNA were 97. 0% and 93. 9%, individually. There was a relatively high accordance among them. There was no significant difference between HBV - DNA and Pre - S1Ag （χ^2 = 2.44, P 〉 0. 05 ）. In the HBeAg negative group, there was also no significant difference between HBV - DNA and Pre - S1Ag （χ^2 =0. 46 ,P 〉0. 05）. As to the Pre - SlAg positive rate, there was significant difference between HBeAg positive group and HBeAg negative group （χ^2 = 343.73,P 〈 0. 05 ）. Conclusion： There is a close and sensitive relation among Pre - S1Ag, DNA and HBeAg, and they are all of great importance to the detection of HBV, showing the reproduction and infectivity of HBV. The Pre -SlAg is of individual detecting value.

关 键 词：乙型肝炎病毒 Pre—S1Ag HBV—DNA HBEAG 

分 类 号：R373.21[医药卫生—病原生物学]