三种纳米材料致大鼠肝肾损伤的初步研究  

Primary study on the hepatotoxicity and nephrotoxicity of rats induced by three kinds of nanomaterials

作　　者：林本成[1] 袭著革[1] 张英鸽[2] 张华山[1] 杨丹凤[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050 [2]国家纳米科学中心军事医学科学院纳米药理毒理协作实验室

出　　处：《卫生研究》2008年第6期651-653,共3页Journal of Hygiene Research

基　　金：国家自然科学基金项目(No.90406024);国家高技术研究发展规划(863)项目(No.2006AA03Z330)

摘　　要：目的研究纳米二氧化硅(Nano-SiO2)、纳米四氧化三铁(Nano-Fe3O4)和单壁碳纳米管(SWCNTs)对大鼠肝、肾损伤作用。方法将42只雄性Wistar大鼠随机分为7组,分别为生理盐水对照组,以及3种纳米颗粒的高剂量组和低剂量组。灌胃染毒4周后处死大鼠,称肝、肾重量,计算肝、肾的脏器系数,测定大鼠血清某些生化指标,并对肝肾组织进行病理学观察。结果各染毒组肝、肾系数与对照组无明显差异;高剂量碳纳米管组碱性磷酸酶(ALP)活性以及高剂量碳纳米管组、高剂量纳米二氧化硅组天门冬氨酸氨基转移酶(AST)活性均较对照组增强(P<0.05);染毒组动物的肝细胞可见有脂肪变性,可见灶性及汇管区炎细胞浸润,各组间无明显差别,肾组织未见明显改变。结论本实验条件下3种纳米颗粒均可对大鼠肝脏产生一定程度损伤作用。Objective To investigate the effects of hepatotoxicity and nephrotoxicity on rats induced by Nano-Si02, Nano-FesO4 and SWCNTs. Methods 42 Wistar rats were randomly divided into the control group, low and high dose groups of three kinds of nanopartieles. The rats were killed when rats were exposed by oral gavage for 4 weeks. The ratios of liver and kidney to body weight, the pathological changes of liver and kidney, serum certain biochemically indexes were detected. Results The ratios of liver and kidney to body weight in experimental groups were not significantly difference in comparison to the control. The ALT and AST level in serum of rats in part experiment groups were more higher than those of the control group. The results of pathological analysis indicated that there were small amounts inflammatory cell infiltration and hepatic cell adipose degeneration in the liver of rats in high-dose group, the change of kidney were not found. Conclusion Three kinds of nanoparticles could induce hepatotoxicity of the rats in the condition of this experiment.

关 键 词：纳米材料 肝毒性 肾毒性 大鼠 

分 类 号：Q593[生物学—生物化学] TB383[一般工业技术—材料科学与工程]