纳米颗粒物对大鼠妊娠结局及其子代近期记忆的影响  

Effect on conception and offspring development in female parenatal rats following intratracheal instillation of nano-C/ZnO and C-ZnO composite nanoparticles

作　　者：张华山[1] 杨丹凤[1] 杨辉[1] 刘焕亮[1] 张伟[1] 林治卿[1] 杨红莲[1] 闫峻[1] 袭著革[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《卫生研究》2008年第6期654-656,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30500399);863项目(No.2006AA03Z330)

摘　　要：目的目的探讨不同化学成分的纳米颗粒物对大鼠妊娠结局及其子代发育的影响。方法将受孕大鼠随机分为4组(纳米碳、纳米氧化锌、纳米碳锌复合颗粒及牛血清对照组),采用气管滴注染毒方式,剂量为7.5mg/kg bw,每天一次,连续5天。待孕鼠自然分娩,记录妊娠率、生产仔鼠数、仔鼠4天存活率及仔鼠行为神经等发育状况。结果纳米氧化锌、碳锌复合颗粒染毒组孕鼠妊娠率分别为57.2%和66.7%,均低于对照组及纳米碳组的100.0%;各组所生产仔鼠均数无显著差异;在哺乳期纳米氧化锌组仔鼠第4、7和21天体重均显著高于其他实验组。水迷宫结果显示,纳米碳锌复合颗粒组在第二、第三潜伏期潜伏时间较其他各组明显增加,纳米碳、纳米氧化锌组的潜伏时间与对照组相比差异无显著性。平面翻正反射、空中翻正反射实验结果与对照组相比差别无显著性。结论该实验条件下,纳米氧化锌对大鼠的生殖有一定的毒性作用,对仔鼠的生长发育有一定的促进作用;纳米碳锌复合颗粒对子代近期记忆有负面影响。Objective To explore the effects of nanoparticles with different chemical components on pregnancy outcome of pregnancy rats and their offspring. Methods Pregnant rats were divided into four groups （ Nano-CB group, Nano-ZnO group, nanoparticles of C-ZnO composite group and Control group） and administered with nanoparticles by intratracheal instillation at the dose of 7.5mg/kg bw once a day for 5 days. After spontaneous delivery, the pregnancy rates, birth numbers, four-day survival rates and neurology development were recorded and analyzed. Results 57.2% and66.7% of pregnancy rates in Nano-ZnO group and Composite nanoparticles group were more lower than those of Control group （100%）. Difference of birth numbers between groups was not significant. Body weights of young rats in Nano-ZnO group at the day of 4, 7 and 21 were more higher than those of other groups. Results of water maze experiment showed that the 2nd and 3rd latent time of Composite nanoparticles group were more longer than those of other groups, while the differences in latent times among Nano-CB group, Nano-ZnO group and Control group were not significant. There was no statically significant difference between particle groups and Control group in experiments of plane righting reflex and air righting reflex. Conclusion Under our experiment condition, Zn0 nanoparticles could exert reproduction toxic effect on rats, whereas could promote the growth and neurology development of offspring. In addition, nanoparticles of C-ZnO Composite could exhibit adverse effect on recent memory of the offspring.

关 键 词：纳米碳 纳米氧化锌 生殖发育毒性 纳米颗粒物 

分 类 号：R994.6[医药卫生—毒理学] Q132.7[医药卫生—药学]