α-甘草酸对药物遗传差异豚鼠血钾浓度的影响  

Effccts of α-Glycyrrhizic Acid on Potassium Concentration in Serum of Difference Pharmacogenetic Hartley Guinea Pig

作　　者：俞进[1] 赖瑛[1] 

机构地区：[1]杭州市中医院药剂科,310007

出　　处：《医药导报》2008年第12期1446-1448,共3页Herald of Medicine

摘　　要：目的探讨α-甘草酸是否导致药物遗传差异豚鼠产生低血钾症。方法体外实验,提取花、白两种豚鼠肾11β-羟基类固醇脱氢酶,研究加入α-甘草酸、β-甘草酸等抑制药后,酶对底物转化率的差异;体内实验,白色豚鼠腹腔注射α-甘草酸15 d后,测定豚鼠肾11β-羟基类固醇脱氢酶活性和血清K+浓度。结果加入α-甘草酸、β-甘草酸等抑制药后,白色豚鼠的11β-羟基类固醇脱氢酶对底物皮质醇的转化率明显低于花鼠(P<0.05);体内实验,白色豚鼠腹腔注射α-甘草酸,导致豚鼠肾11β-羟基类固醇脱氢酶活性下降和血清K+浓度降低,但150 mg.kg-1剂量组未致白色豚鼠产生低血钾症。结论花、白两种豚鼠之间存在药物遗传差异;常规剂量的α-甘草酸不会导致白色豚鼠产生低血钾症。Objective To study the effects of α-glycyrrhizic acid on hypokalemia in difference pharmacogenetic hartley guinea pig. Methods In vitro, 11β-hydroxysteroid dehydrogenase was extracted from white and muhicoloured hartley guinea pig kidney and mixed with inhibitory agents, such as α-glycyrrhizic acid. The conversion percentage of cortisol was determined. In vive, α-Glycyrrhizic acid was injected intraperitoneally during total 15 days. The activity of 11β-hydroxysteroid dehydrogenase from hartley guinea pig kidney and potassium concentration in serum were measured. Results In vitro, compared to muhicoloured hartley guinea pig, 11 -hydroxysteroid dehydrogenase in white one exhibited a lower conversion percentage of cortisol（P 〈 0. 05 ）. In rive, the activity of 11β-hydroxysteroid dehydrogenase from hartley guinea pig kidney and potassium concentration in serum were reduced significantly. But 150 mg ·mg^-1 α-glycyrrhizic acid, a normal dose, did not induce hypokalemia in white hartley guinea pig. Conclusion There is significant difference of pharmacogenetics between white and muhicoloured hartley guinea pig.

关 键 词：Α-甘草酸 脱氢酶 低血钾症 药物遗传 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]