降落PCR扩增单纯疱疹病毒Ⅱ型G蛋白基因的研究  

Detection of gG Gene of Herpes Simplex Virus Type Ⅱ by TD-PCR

作　　者：刘涛[1] 黄志成[1] 祝水芬[1] 

机构地区：[1]杭州市疾病预防控制中心微生物检验科,浙江310006

出　　处：《中国预防医学杂志》2008年第11期963-965,共3页Chinese Preventive Medicine

摘　　要：目的建立一种快速,准确检测单纯疱疹病毒Ⅱ型G蛋白基因的方法。方法根据已发表的gG蛋白核苷酸序列设计引物,采取降落PCR对gG蛋白基因进行扩增。结果从2份病毒株中均扩增出与预期大小完全一致的目的片段,并且能有效地降低或避免非特异性扩增。结论TD-PCR能够用于快速,准确地检测gG蛋白基因,为生殖器疱疹的鉴别诊断在分子水平上提供了理想的新方法。Objective To establish a method that can instantly and precisely test the protein G gene of herpes simplex virus type Ⅱ. Methods Primer was designed according to the published protein G nucleotide sequence, and the DNA of protein G was amplified with the touchdown PCR （TD - PCR）. Results The target fragments were amplified from two herpes simplex virus type Ⅱ strains and were exactly the same as expected. The method could reduce or avoid the non-specific amplification effectively. Conclusion The TD-PCR can be used for instant and precise test of protein G. It is a new and ideal method for genital herpes diagnosis on molecular level.

关 键 词：TD-PCR SG蛋白 单纯疱疹病毒Ⅱ型 

分 类 号：R450[医药卫生—治疗学]