登革3型病毒07CHLS001株全基因组序列测定和分析  

Complete Sequence Determination and Analysis of the Dengue-3 Virus 07CHLS001 Strain

作　　者：雷永良[1] 陈燕飞[1] 叶碧峰[1] 梅建华[1] 陈秀英[1] 兰进权[1] 李永芬[1] 

机构地区：[1]丽水市疾病预防控制中心,浙江323000

出　　处：《中国预防医学杂志》2008年第11期967-971,共5页Chinese Preventive Medicine

摘　　要：目的对登革3型病毒浙江分离株07CHLS001进行了全基因组序列测定和分析,为探讨其基因组特征和来源提供依据。方法根据登革3型病毒98TW407株设计22对引物,利用RT-PCR法扩增出07CHLS001株基因组不同区段的cDNA片段,通过序列测定和拼接获得其全基因组序列。结果07CHLS001株基因组全长10 707 nt,包含一个开放读码框(95-10 267 nt),编码3 390个氨基酸。它与登革3型病毒ThD3-1687-98株、98TW407株和80-2株的全基因组序列核苷酸相似性依次为98.7%、98.5%和94.6%。prM/M-E核苷酸序列系统发生树分析表明,07CHLS001与来自泰国的5个株系形成了一个Bootstrap支持率为90%的分枝。结论07CHLS001属于登革3型病毒的亚型Ⅱ。07CHLS001株全序列的测定,对进一步研究该株系的特性有十分重要的意义。Objective To understand the genomic characteristics and the origin of the dengue -3 virus 07CHLS001 strain. Methods Twenty two primer pairs were designed according to the sequence of dengue - 3 virus 07CHLS001 strain. The cDNAs of different regions of 07CHLS001 genomie RNA were amplified by RT - PCR The sequences of cDNAs were determined and assembled. Results 07CHLS001 genomic RNA consists of 10 707 nucleotides and contains a single open reading frame （95 - 10 267 nt）which encoded a polyprotein of 3 390 amino acids residues. Compared with other dengue -3 virus strains ThD3 - 1687 - 98, 98TW40 and 80 - 2, the complete nucleotide sequence homology was 98.7%, 98.5% and 94. 6% , respectively. Phylogenetic analysis of the nucleotide sequence of prM/M - E revealed that 07CHLS001 formed a clade with five strains from Thailand with Bootstrap support of 90%. Conclusion 07CHLS001 belongs to dengue -3 virus subtype Ⅱ. The determining of the complete sequence of genomic RNA would aid study of the characteristics of 07CHLS001.

关 键 词：登革病毒 3型 基因组 序列分析 

分 类 号：R450[医药卫生—治疗学]