蛇莓乙酸乙酯萃取物的化学成分  

Study on chemical constituents of Duchesnea indica Andr.Focke

作　　者：苗青[1,2] 包海燕[1,2] 朴淑娟[1] 林厚文[1] 邱峰[2] 

机构地区：[1]第二军医大学长征医院药学部,上海200003 [2]沈阳药科大学中药学院天然药物化学教研室,沈阳110016

出　　处：《第二军医大学学报》2008年第11期1366-1370,共5页Academic Journal of Second Military Medical University

摘　　要：目的:研究蛇莓乙酸乙酯萃取物的化学成分。方法:以95%乙醇回流提取,对提取液的乙酸乙酯萃取物采用正相硅胶、反相硅胶、Sephadex LH-20柱色谱和高效液相色谱进行分离纯化,并根据理化性质和波谱数据鉴定化合物的化学结构。结果:分离得到13个化合物,分别鉴定为蔷薇酸(1),arjunic acid(2),对羟基桂皮酸(3),芹菜素(4),山柰酚(5),2α-羟基乌苏酸(6),2α-羟基齐墩果酸(7),委陵菜酸(8),刺梨苷(9),翻白叶苷A(10),野蔷薇苷(11),紫云英苷(12)和异槲皮苷(13)。结论:化合物2～5、10、12均为首次从蛇莓属中分离得到。Objective： To investigate the chemical constituents in the EtOAc fraction of the EtOH extract of Duchesnea indica Andr. Focke. Methods： Extraction was done with 95% EtOH. EtOAc fraction of the EtOH extract was isolated and purified by column chromatography using silica gel,RP-C18 ,Sephadex LH-20 and HPLC. The structures of the compounds were identified by their physico-chemical properties and spectral analysis. Results： Thirteen compounds were obtained from the EtOAc fraction of Duchesnea indica Andr. Focke and they were identified as euscaphic acid（1）, arjunic acid（2）, p hydroxy cinnamic acid （3）, apigenin（4 ）, kaempferol （ 5 ）, 2 α-hydroxy ursolic acid （ 6 ）, 2 α-hydroxy oleanolic acid （ 7 ）, tomentic acid （ 8 ）, kaii-ichigeside F1 （9）, potengriffioside A （ 10 ）, rosamultin （ 11 ）, kaempferol-3-O-β-D-glucoside （ 12 ）, and isoquercitrin （ 13 ）. Conclusion： The compounds 2-5,10,and 12 have been isolated from the genus of Duchesnea for the first time.

关 键 词：蛇莓 分离与纯化 中药鉴定 芹菜素 翻白叶苷A 

分 类 号：R284.1[医药卫生—中药学]