东北红豆杉茎尖组织培养  

The Culture of New Bud Tissue of Taxus cuspidata

作　　者：程广有[1] 唐晓杰[1] 杨振国[1] 

机构地区：[1]吉林林学院森林资源系

出　　处：《吉林林学院学报》1997年第4期209-211,共3页

基　　金：吉林省教委资助

摘　　要：以ＭＳ培养基为基础加入不同质量浓度的吲哚丁酸（ＩＢＡ）和６苄基氨基腺嘌呤（６ＢＡ），对东北红豆杉茎尖进行组织培养．结果表明，茎尖生长量和愈伤组织生长量因激素质量浓度不同而不同，６ＢＡ／ＩＢＡ比值大时，可产生不定芽．从２年生植株上采取的茎尖产生不定芽的百分率较１７年生的高．当ＩＢＡ质量浓度大时，茎尖容易形成不定根，从２年生植株上取的茎尖形成不定根的百分率大于１７年生的植株上取的茎尖．New buds of Taxus cuspidata were cultured with different quality concentrations of IBA and 6 BA.The results showed,the growth of new buds and callus are different with hormon quality concentration.When the specific value of 6 BA/IBA was bigger,many adventitious buds growed up.The percentage of adventitious buds from 2 year old plants is higher than from 17 year old plants.When the quality concentration of IBA was higher,adventitious roots growed up easily.The percentage of adventitious roots from 2 year old plants is higher than from 17 year old plants.

关 键 词：红豆杉 茎尖 组织培养 

分 类 号：S791.490.5[农业科学—林木遗传育种]