二肽基肽酶Ⅳ与卵巢上皮性癌临床病理特征和预后的关系  

Correlation of DPPⅣ expression with clinicopathological features and prognosis in epithelial ovarian carcinoma

作　　者：张梦真[1] 乔玉环[1] 索振河 

机构地区：[1]郑州大学第一附属医院妇产科,450052 [2]河南省高校临床医学重点开放实验室

出　　处：《中华肿瘤杂志》2008年第11期848-852,共5页Chinese Journal of Oncology

摘　　要：目的探讨卵巢上皮性癌组织中二肽基肽酶Ⅳ（DPPⅣ）的表达及临床意义。方法采用免疫组化法检测378例卵巢上皮性癌组织中DPPⅣ蛋白的表达，采用原位杂交法检测86例卵巢上皮性癌组织中DPPⅣ mRNA的表达，42例正常卵巢组织作为对照。结果DPPⅣ蛋白在卵巢上皮性癌组织中的总阳性表达率为92．9％（351／378），在正常卵巢组织中的总阳性表达率为59．5％（25／42），差异有统计学意义（P=0．001）。DPPⅣ蛋白的表达与患者的发病年龄、肿瘤分化程度和FIGO分期无关（均P〉0．05），而与肿瘤的组织学类型有关（P=0．005）。DPPⅣ蛋白的表达水平越高，患者的生存期越短（P=0．023）。DPPⅣ mRNA在卵巢上皮性癌组织中的总阳性表达率为97．7％（84／86），在正常卵巢组织中的总阳性表达率为64．3％（27／42），差异有统计学意义（P〈0．05）。卵巢上皮性癌组织中DPPⅣ蛋白和mRNA的表达与定位一致，且二者呈正相关（P=0．001）。结论DPPⅣ在卵巢上皮性癌的发生发展中起着一定作用，其表达水平可能成为影响患者预后的因素。Objective To investigate the expression of dipeptidyl peptidase Ⅳ （ DPPⅣ ） in patients with epithelial ovarian carcinoma （EOC） and its clinical significanee. Methods Immunohistoehemistry （IHC） was used to detect the expression of DPP Ⅳ protein in 378 formalin-fixed paraffin-embedded EOC tissue samples. The expression of DPPⅣ mRNA in 86 EOC tissue samples were examined by in situ hybridization （ISH） using specific FITC-labelled RNA probes. Forty-two samples of normal ovarian tissues were used as control. Statistical analyses were carried out by Chi-square test, Spearman rank correlation and Kaplan-Mejer method. Results Among the 378 epithelial ovarian carcinomas, 351 （92.9%） showed a positive expression of DPP 1V protein, while only 25/42 （ 59.5% ） of normal ovaries had a positive expression by semiquantitative IHC analysis. The expression level of DPP Ⅳ protein was significantly lower in the normal ovaries than that in ovarian carcinomas （χ^2 = 18.4 ,P = 0. 001 ）. There was no significant correlation between the expression of DPP Ⅳ protein and age, FIGO stage and histological grade （P 〉 0.05）. However, the expression of DPPⅣ protein was significantly associated with histological type （χ^2 = 28.5, P = 0.005）. The patients with high level expression of DPPⅣ protein likely had a poor prognosis in terms of overall survival （ P = 0.02 ）. Of the 86 patients, 84 （97.7%） showed positive expression of DPPⅣ mRNA, also higher than that in nomkal ovarian tissues （P 〈0.05）. A statistically significant correlation between DPPⅣ mRNA and protein expression was observed （rs=0. 66, P =0.001 ）. Conclusion DPPⅣ may be involved in the carcinogenesis of ovarian cancer, and may become a potential prognostic marker for epithelial ovarian carcinoma.

关 键 词：卵巢肿瘤 二肽基肽酶Ⅳ 原位杂交 CRNA探针 预后 

分 类 号：R686[医药卫生—骨科学]