白癜风患者皮损中CD4^+,CD8^+T淋巴细胞及朗格汉斯细胞的检测  

Detection of CD4^+,CD8^+T Cells and Langerhans Cells in Lesions of Patients with Vitiligo

作　　者：张继玲[1,2] 曹碧兰[1] 袁伟[1] 陈晓红[1] 刘华庆[3] 杨宪鲁[1] 汪治国[1] 

机构地区：[1]遵义医学院附属医院皮肤科,贵州遵义563000 [2]江苏省邳州市人民医院皮肤科,江苏邳州221300 [3]遵义医学院附属医院病理科,贵州遵义563000

出　　处：《中国皮肤性病学杂志》2008年第11期649-651,共3页The Chinese Journal of Dermatovenereology

基　　金：贵州省卫生厅资助项目(02-D-176)

摘　　要：目的探讨白癜风患者皮损中CD4+,CD8+T淋巴细胞、朗格汉斯细胞(LC)及黑素细胞(MC)在白癜风发病中的作用。方法采用Envision免疫组化染色法,对12例白癜风进展期患者和9例稳定期患者皮损处CD4+,CD8+T淋巴细胞、LC及MC进行检测,并与10例正常人皮肤进行对照。结果白癜风患者皮损中CD4+,CD8+T淋巴细胞、LC表达较对照组显著增多(P<0.01),而MC表达较对照组显著减少(P<0.01)。结论白癜风患者皮损中LC,CD4+,CD8+T淋巴细胞异常表达可能参与白癜风的发病,其作用模式可能是LC抗原递呈,CD4+,CD8+T淋巴细胞浸润破坏或攻击MC,从而引起白癜风患者表皮基底层的MC减少或消失,导致白癜风的发生。Objective To study CD4^＋ , CD8^＋ T cells, Langerhans cells and Melanocytes in lesions of patients with vitiligo and to investigate the role and relationship of them in the pathogenesis of vitiligo. Methods The expression of CD4^＋ , CD8^＋ T cells, Langerhans cells and Melanocytes was detected by immunohistochemistry（ Envision method）in vitiliginous lesions of 12 patients with active vitiligo and 9 patients with static vitiligo, and 10 normal controls. Results The expression of CD4^＋ , CD8^＋ T cells and Langerhans cells was significantly increased in lesions of patients with vitiligo compared to those in normal controls （P 〈 0.01 ）, and also the expression of melanocytes was obviously decreased in lesions of patients with vitiligo compared to those in normal controls（P 〈 0.01 ）. Conclusion The abnormal expression of CD4^＋, CD8^＋T cells and Langerhans cells in vitiliginous skin is probably related of pathogenesis of vitiligo, their function in local immune reactivity is probably infiltration of T ceils actived by Langerhans ceils presenting antigen, resulting in the destruction of Melanocytes.

关 键 词：白癜风 CD4^+T淋巴细胞 CD8^+T淋巴细胞 朗格汉斯细胞 黑素细胞 

分 类 号：R758.4[医药卫生—皮肤病学与性病学]