禽流感病毒NS1蛋白对细胞的影响  

Effect of Influenza Virus NS1 Protein on Host Cell

作　　者：赵莉霞[1] 张莹莹[1] 杨志新[1] 许龙[1] 杨予涛[1] 于孟斌[1] 王荣[1] 周晓巍[1] 黄培堂[1] 

机构地区：[1]军事医学科学院生物工程研究所蛋白质工程实验室,北京100071

出　　处：《生物工程学报》2008年第11期1912-1917,共6页Chinese Journal of Biotechnology

基　　金：国家科技支撑计划项目(No.2006BAD06A01)资助~~

摘　　要：NS1蛋白为流感病毒非结构蛋白,只在病毒侵入宿主细胞后产生。目前NS1蛋白对细胞整体水平上的作用仍不清楚,为了解NS1蛋白在病毒感染细胞中的作用,构建了重组质粒pCMV-myc-NS1并将其转染A549细胞,利用双向电泳技术检测了受NS1蛋白调控的宿主蛋白,以期从蛋白质组水平上研究禽流感病毒与宿主细胞间的相互作用。同时,还检测了转染NS1对细胞增殖和细胞周期的影响。结果显示,NS1在细胞中的表达,能够明显引起宿主细胞代谢的变化,并通过阻滞细胞周期的正常进行而减缓细胞的增殖。NS1 is a non-structural protein of the influenza A virus, which could only be expressed when cells are infected. The effect of NS1 protein on host cell is still not clear. To understand the role of NS1 protein in cell infection, recombinant plasmid pCMV-myc-NS 1 was constructed, and then transfected into A549 cells. Two-dimensional electrophoresis was employed to analyze proteins regulated by NS 1 that could reflect the interaction between influenza virus and host cells at the protein level. The influence of NS1 on cell proliferation and cell cycle was also studied. The result showed that not only could NS 1 remarkably affect metabolism but it could also slow down cell proliferation through blocking cell cycle.

关 键 词：NS1蛋白 宿主细胞 双向电泳 细胞增殖 细胞周期 

分 类 号：R373[医药卫生—病原生物学]