IL-10降低大鼠急性坏死性胰腺炎IL-1β释放  

IL-10 reduces Interleukin-1β in rats with acute necrotizing pancreatitis

作　　者：邓德海[1] 梁志海[1] 唐国都[1] 

机构地区：[1]广西医科大学第一附属医院消化内科,广西南宁530021

出　　处：《基础医学与临床》2008年第11期1160-1163,共4页Basic and Clinical Medicine

基　　金：广西自然科学基金(桂科自0447054)

摘　　要：目的探讨重组人白介素10(IL-10)对大鼠急性坏死性胰腺炎(ANP)血清IL-1β的影响。方法健康雄性SD大鼠92只,随机分成对照组(C组)、ANP组(A组)和IL-10干预组(I组)。A组大鼠腹腔内注射6%的左旋精氨酸(L-Arginine)1.0mg/g体重,共3次,每次间隔1h,诱导ANP;I组于L-Arginine注射诱导胰腺炎后2、5和8h分别腹腔内注射重组人IL-101万U,共3万U;C组大鼠给予0.9%生理盐水。在诱导胰腺炎后第4、12、24和36h共4个时点检查胰腺组织,用酶联免疫吸附法(ELISA)检测血清淀粉酶水平、血清IL-1β水平。结果A组各时点IL-1β、血清淀粉酶水平以及胰腺组织病理学评分较C组明显增高(P<0.05或P<0.01);其中,胰腺组织病理学评分及血清淀粉酶升高以24、36h最为显著;IL-1β则以4、12h升高明显;而I组组织病理学评分(除4h外)、血清淀粉酶、IL-1β各时点值均显著低于A组(P<0.05或P<0.01)。结论早期应用重组人IL-10可以抑制炎症细胞因子IL-1β释放,降低炎症反应的严重程度,减轻实验性胰腺炎的病理损害。有可能是治疗ANP的新途径。Objective To investigate the effects of recombinant human interleukin（IL）-10 on serum interleukin-1β in L-arginine-induced acute necrotizing pancreafitis of rats. Methods Ninty-two Sprague-Dawley rats were randomly divided into three groups. Group A（ n = 36） and Group I（ n = 32） received three intraperitoneal injections of 6% L-arginine （1.0 mg/g） at hourly intervals. Group I（ n = 32） was treated with 10,000 unites of intraperitioneal recombinant human IL-10 at the 2nd, 5th and 8th hour after the last injection of L-arginine. Group C （n = 24） received saline alone. Rats were killed at the 4th, 12th, 24th and 36th hour after the last L-arginine injection. Serum intedeukin-1 β and amylase were assayed. Pancreatic tissues were examined histopathologically by microscopy. Results Serum amylase, interleukin-1β and pancreatic histopathologieal scores in group A increased significantly after L-arginine injection（P 〈0. 05 or P 〈0. 01 ）. The level of serum amylase and interleukin-1β in group I were significantly decreased at various time points （P 〈0. 05 or P 〈0. 01 ）. Pancreatic histopathological scores in group I decreasedcomparewithgroupAatthe12th,24th ,36thhour（P 〈0. 05orP 〈0. 01 ）. Conclusion IL-10 alleviates the severity of experimental acute necrotizing pancreatitis by inhibiting of interleukin-1 β release.

关 键 词：胰腺炎 急性 白介素10 白介素1Β 淀粉酶 

分 类 号：R657.51[医药卫生—外科学]