感染性休克大鼠内源性Apelin及其受体系统表达下调  

Down regulation on endogenous apelin and its receptor expression in the rats with septic shock

作　　者：薛林[1] 潘春水[1] 滕旭[2] 赵春玉[1] 蔡嫣[2] 唐朝枢[1] 齐永芬[1,2] 

机构地区：[1]北京大学第一医院心内科,北京100034 [2]北京大学医学部生理与病理生理学系,北京100083

出　　处：《基础医学与临床》2008年第11期1183-1186,共4页Basic and Clinical Medicine

摘　　要：目的观察内源性Apelin及其受体(血管紧张素受体AT1相关蛋白,APJ)在盲肠结扎和穿孔所致感染性休克大鼠的变化。方法20只雄性Wistar大鼠,体重220～250g,随机分为假手术对照组和晚期休克组。酶联免疫法测定血浆和心肌中Apelin含量,RT-PCR法测定心肌组织Apelin、APJ mRNA水平,Western blot方法测定心肌组织中APJ蛋白水平。结果与假手术组比较,休克组大鼠+LVdP/dtmax和-LVdP/dtmax明显降低;左心室舒张末期压(LVEDP)增高,平均动脉压降低,心率增快;有严重低血糖症和高乳酸血症。血浆和心肌Apelin含量明显降低(P<0.01),心肌组织Apelin、Apelin受体APJ mRNA水平显著下降(P<0.01),心肌组织APJ蛋白水平明显降低(P<0.01)。结论Apelin及其受体系统下调在感染性休克发生发展中可能有重要作用。Objective To observe the variation of endogenous Apelin with its APJ receptor in rats model with septic shock caused by cecal ligation and puncture （CLP）. Methods Twenty male Wistar rats weighting 200 - 250 g randomized into two groups, control group and severe septic shock group. Enzyme immunoassay （EIA） was used to detect Apelin in plasma and myocardia. RT-PCR was used to analyze the relative amount of mRNA of Apelin and APJ in the myocardia. Western blot was used to measure the protein level of myocardial APJ. Results Compared with sham, rats with sepsis showed inhibited cardiac LVEDP elevated）. Rats with septic shock showed function （ ＋ LVdP/dtmax and -LVdP/dtmax decreased, and significant hypoglycemia and hyperlacticaemia, lower level of Apelin in plasma and myocardia, decreased mRNA relative amount of Apelin and APJ in the myocardia, and lower myocardial APJ protein level. Conclusion Apelin and its receptor play an important role in pathogenesis of septic shock.

关 键 词：感染性休克 APELIN APJ 心血管 

分 类 号：R459.7[医药卫生—急诊医学]