脂肪酸生物标记法研究零排放猪舍基质垫层微生物群落多样性  

The diversity of PLFAs biomarkers for the microbial community in the stroma cushion of non-pollution pigsty

作　　者：刘波[1] 郑雪芳[1] 朱昌雄[2] 蓝江林[1] 林营志[1] 林斌[3] 叶耀辉 

机构地区：[1]福建省农业科学院农业生物资源研究所,福州350003 [2]中国农业科学院环境与可持续发展研究所,北京100081 [3]福建省农业科学院农业工程研究所,福州350003 [4]福建省宁德地区农科所,宁德355003

出　　处：《生态学报》2008年第11期5488-5498,共11页Acta Ecologica Sinica

基　　金：国家水体污染控制与治理科技重大专项资助项目(2008ZX07425-002);福建省财政专项资助项目--福建省农业科学院科技创新团队建设基金(STIF-Y03);福建省发展和改革委员会饲用益生菌产业化技术研究资助项目~~

摘　　要：用微生物群落脂肪酸生物标记总量,分析零排放猪舍基质垫层微生物群落的数量变化,日本洛东微生物菌种处理组和零排放I号菌种处理组各层微生物脂肪酸生物标记含量变化趋势相近。基质垫层微生物群落脂肪酸生物标记检测出37个生物标记,构成微生物群落的指纹图谱,含量最高的前4个生物标记为:细菌16:00含量为431260,细菌18:1ω9c含量为413075,厌氧细菌18:1ω7c含量为101368,耗氧细菌i15:0含量为90328.不同的生物标记多样性指数在基质垫层不同层次分布不同,可作为衡量特定微生物生物标记功能的一个指标。通过基质垫层微生物脂肪酸生物标记特征指数B的分析,提出了微生物群落分布的特征指标,生物标记特征指数B越高,表明微生物群落中的细菌和真菌含量越高,有利于基质垫层分解粪便排泄物,可作为基质垫层微生物群落变化优劣的特征性指标;通过基质垫层耗氧细菌和厌氧细菌脂肪酸生物标记含量比值,构建发酵指数F,作为基质垫层发酵特性的指标,发酵指数F越高,表明耗氧细菌起的作用越大,反之,耗氧细菌起的作用越小,生物标记的发酵指数可以作为研究粪便排泄物的微生物分解过程的指数。The PLFAs biomarkers were introduced into analyzing the The diversity of PLFAs biomarkers for the microbial community in the stroma cushion of non-pollution pigsty. The microbial strains of LUODON and LPF #1 were used to treat the stroma cushion in the non-pollution pigsty. The results showed that the efficiency of fomentation on the pig effluent by the two strains were similar. 37 of PLFAs biomarkers were detected out by the MIDI microbial identify system, which constructed the finger printing of the microbial community in the stroma cushion in the non-pollution pigsty. The four most high of PLFAs were the bacteriun biomarker of 16：00 with 431260, bacterium hiomarker of 18：1 009c with 413075, the anaerobic bacterium biomarker of 18：1 ω7c with 101368,the aerobic bacterium of i15：0 with 90328. The different PLFAs biomarkers were distributed in the different layers of stroma cushion with different densities. The new characteristic index （B） of the PLFAs biomarkers was built up as B = [ PLFAs bacterium PLFAs fungus]/[ PLFAs actinomycetes PLFAs protest ] to analyze the structure of microbial community. The new fermentation index （F） of the PLFAs hiomarkers was built up as F = [ aerobic bacterium PLFAs/anaerobic bacterium PLFAs ] to distinguish the fermentation state in the stroma cushion of pigsty.

关 键 词：零排放猪舍 基质垫层 磷脂脂肪酸(PLFAs) 

分 类 号：S852.6[农业科学—基础兽医学] S828[农业科学—兽医学]