基于ISSR标记的扁玉螺(Neverita didyma)自然居群遗传结构  

Genetic structure of natural population of Neverita didyma based on ISSR markers

作　　者：孙始威[1] 孙振兴[1] 葛宜和[1] 闫冬春[1] 黄清荣[1] 

机构地区：[1]鲁东大学生命科学学院,烟台264025

出　　处：《生态学报》2008年第11期5499-5505,共7页Acta Ecologica Sinica

基　　金：山东省自然科学基金资助项目(Y200D12)~~

摘　　要：利用ISSR分子标记技术,对采自大连(DL)、烟台(YT)及青岛(QD)近海的扁玉螺3个自然居群的遗传结构和遗传多样性进行了分析。用13个引物对90只个体进行了PCR扩增,共检测到161个位点,3个居群的多态位点比例为74.53%～85.09%,各居群遗传多样性水平的高低依次为YT>QD>DL。扁玉螺在物种水平上的Nei’s基因多样性指数和Shannon’s信息指数分别为0.3395和0.5113,在居群水平上分别为0.2811和0.4189,显示出扁玉螺有着较高的遗传多样性。AMOVA分子变异分析表明,扁玉螺的遗传变异有27.16%发生在居群间,72.84%发生在居群内,居群内的遗传变异大于居群间的遗传变异。扁玉螺3个居群间的遗传分化系数(Gst)为0.1720,基因流(Nm)为2.4063,Nei’s遗传距离平均值为0.1228,表明扁玉螺居群间虽然存在着一定程度的遗传分化,但仍属于种内正常分化的范畴。上述结果为保护和利用扁玉螺资源提供了科学依据。The genetic structure and genetic diversity in three natural populations of Neverita didyma were analyzed by using the molecular marker technique of inter simple sequence repeat （ISSR）. The samples of the three populations were taken from the offshores of Dalian （ DL）, Yantai （YT） and Qingdao （ QD）, respectively. A total of 161 loci from 90 individuals were polymerase-chain-reaction （PCR） amplified with 13 primers. The proportion of polymorphic loci in the three populations ranged from 74.53 % to 85.09 %. The abundance of genetic diversity in the three populations arranged in a decent order was YT 〉 QD 〉 DL. The Nei＇s gene diversity and Shannon＇s information index of Neverita didyma was 0. 3395 and 0. 5113 at species level, 0. 2811 and 0. 4189 at population level, respectively, showing that the genetic diversity of Neverita didyma was relatively high. An analysis of the molecular variance （AMOVA） demonstrated that the among-population component accounted for 27. 16 % of the total variation, while the within-population component accounted for 72.84 %. The within-population genetic variation was apparently larger than the among-population. The genetic differentiation coefficient （Gst）, gene flow （Nm） and the average Nei＇s genetic distance were 0. 1720, 2. 4063 and 0. 1228, respectively among the three populations. The results indicate that although there is genetic differentiation of some extent among the three populations of Neverita didyma, such differentiation is well contained in the context of normal differentiation within the species. The results have laid the scientific basis for the conservation and utilization of the Neverita didyma resources.

关 键 词：扁玉螺 遗传结构 ISSR 

分 类 号：S917.4[农业科学—水产科学]