抗ds—DNA抗体、抗ENA多肽抗体联合检测在SLE诊断中的应用  

Clinical Diagnostic Value of Collective Determination of Anti - ds - DNA and Anti - ENA Polypeptide Antibody for SLE

作　　者：王泉[1] 范友谊[1] 赵建斌[1] 

机构地区：[1]徐州市第一人民医院检验科,江苏徐州221002

出　　处：《现代保健（医学创新研究）》2008年第21期7-8,共2页

摘　　要：目的为探讨抗ds—DNA抗体、抗ENA多肽抗体联合检测在系统性红斑狼疮（SLE）诊断中的应用。方法应用抗ds—DNA抗体（间接免疫荧光法IIF）、抗ENA多肽抗体试剂盒（免疫印迹法IBT）分别检测抗ds—DNA抗体及抗ENA多肽抗体谱。结果156例SLE患者中共检出抗ds—DNA抗体阳性98例（62．8％），抗ENA多肽抗体阳性112例（71．8％），SLE患者组抗ds—DNA抗体及抗ENA多肽抗体指标均高于正常对照组（P〈0．01）。结论抗ds—DNA抗体及抗ENA多肽抗体在SLE诊断中具有互补作用，并能提高对SLE诊断符合率，在观察治疗及预后中具有重要的临床价值。Objective To investigate the application of collective determination of anti - ds - DNA and anti - ENA polypeptide antibody in diagnosis of SLE patients. Methods To test anti - ds DNA by indirect immunoflorescence while to test ENA polypeptide antibody by immunolabelling technique（IBT）. Results In 156 cases SLE test. anti - ds - DNA postitive rate was 62. 8% （98 cases） and anti - ENA polypeptide antibody positive rate was 71.8% （112 cases）. It was showed that positive rates of anti -ds -DNA and anti -ENA polypeptide antibody were significantly higher in SLE patients than those in normal controls. Conclusions In diagnosing SLE, collective determination of anti - ds DNA and anti - ENA polypetide antibody can complement each other upgrade the detectable rate and have the significant clinical value in diagnostic classification and prognosis of the diseases.

关 键 词：抗ds—DNA抗体 抗ENA多肽抗体 系统性红斑狼疮 

分 类 号：R446.6[医药卫生—诊断学]