提高不同苜蓿品种体细胞胚发生潜力的研究  

Improvement on the Potential of Somatic Embryogenesis in Different Alfalfa Cultivars

作　　者：王玉民[1] 刘艳芝[1] 于淑梅[1] 姜昱[1] 谭化[1] 董英山[1] 

机构地区：[1]吉林省农业科学院,长春130033

出　　处：《牧草与饲料》2008年第4期11-13,共3页Journal of Forage & Feed

摘　　要：在本项研究中，以来自中国东北和内蒙古地区7个商业苜蓿品种为材料，以无菌苗的子叶和叶片作为外植体，对其体细胞胚发生进行了研究。在我们所用的组织培养程序中，所有品种均有体外胚发生，但品种之间形成体细胞胚的能力差别很大。以从子叶和叶片再生植株的叶片为外植体，体细胞胚发生率明显提高，7个品种的平均值分别为73．01％和85．40％。由于体细胞胚诱导效率大大提高，建议在对再生频率低的优良苜蓿品种进行遗传转化时，使用再生植株叶片为外植体。In this study, the invitro embryogenic responses of seven commercial alfalfa cultivars in the northeast and Inner Mongolia areas of China were tested using cotyledon and leaf as explants. All the cultivars gave positive responses in our tissue culture procedure and the ability of forming somatic embryos varied greatly amongst cultivars. The frequencies of somatic embryogenesis of regenerated plant leaves from both cotyledons and leaves were significantly improved, with a mean of 73.01% and 85.40% respectively. Because of its high efficiency of somatic embryo induction, it is suggested that regenerated plant leaf be used as explants in genetic transformation of alfalfa, especially for the elite cuhivars of low regeneration frequency.

关 键 词：苜蓿 组织培养 体细胞胚发生 植株再生 

分 类 号：S541[农业科学—作物学]