HLA衍生肽RDP1258对大鼠淋巴细胞增殖活性的影响  

Effect of HLA-derived peptide RDP1258 on proliferation of rat T lymphocytes

作　　者：崔志刚[1] 汪泽厚[2] 岳明[1] 叶钢[3] 黄赤兵[3] 张艮甫[3] 

机构地区：[1]解放军第306医院泌尿外科,北京100101 [2]解放军空军总医院泌尿外科,北京100036 [3]第三军医大学附属新桥医院泌尿外科,重庆400036

出　　处：《国际检验医学杂志》2008年第11期961-963,共3页International Journal of Laboratory Medicine

基　　金：国家新药基金资助项目(No.9690105184)

摘　　要：目的探讨合成HLA衍生肽RDP1258对T细胞活化、增殖活性的影响。方法采用Fmoc固相化学合成法人工合成RDP1258,MTT法分别进行大鼠淋巴细胞增殖试验、大鼠单向混合淋巴细胞反应(MLR)及人外周血淋巴细胞毒试验。结果合成RDP1258纯度达95%以上,相对分子质量与理论值相符;RDP1258显著抑制刀豆蛋白A(ConA)刺激的淋巴细胞增殖;RDP1258能抑制单向MLR反应体系中应答细胞对刺激细胞的反应能力。结论RDP1258体外能抑制由丝裂原或同种异体抗原引起的大鼠淋巴细胞增殖反应。Objective To investigate the effects of RDP1258 on T lymphocyte activation and proliferation. Methods RDP1258 was synthesized by using Fmoc solid phase chemosynthesis method. MTT reduction assay was applied to performing rat lymphocyte proliferation assay, rat one-way mixed lymphocyte reaction and human peripheral blood lymphocyte cytotoxicity test. Results The purity of synthesized RDP1258 was above 95%. The molecular weight was 1 229.9 Da in accord with the theoretical value. RDP1258 could inhibit proliferation response of rat T lymphocyte to ConA in a dose-dependent manner and rat mixed lymphocyte reaction. Conclusion RDP1258 can in vitro inhibit proliferation response of rat T lymphocyte to mitogen or to alloantigens.

关 键 词：肽类 淋巴细胞 细胞增殖 

分 类 号：R392[医药卫生—免疫学]