蜜蜂微孢子虫在中国的自然种系构成初探  

Natural stripe of Microsporidia of honeybee in China

作　　者：刘锋 王强[1] 代平礼[1] 吴艳艳[1] 宋怀磊[1] 周婷[1] 

机构地区：[1]中国农科院蜜蜂研究所农业部授粉昆虫生物学重点开放实验室,北京100093

出　　处：《昆虫知识》2008年第6期963-966,共4页Entomological Knowledge

基　　金：科技部自然资源平台项目(2007CB109203);十一五支撑项目(2006BAD06B04);科技部农业公益性行业科研专项(nyhyzx07-041)

摘　　要：蜜蜂微孢子虫(Nosema apis及Nosema ceranae)是微孢子虫的典型代表之一,由它寄生蜜蜂所产生的疾病,称为蜜蜂微孢子虫病。通过目前国际上普遍使用的克隆16srRNA基因(16srDNA)的片段并测序的方法来进行蜜蜂微孢子虫在我国主要养蜂地区的分布情况。结果表明,在我国主要养蜂地区,造成大量西方蜜蜂患蜜蜂微孢子虫病的是东方蜜蜂微孢子虫Nosema ceranae,至今为止未发现过去我们广泛认定的西方蜜蜂微孢子虫Nosema apis。Microsporidia is a kind of protist that parasitizes in the cell.Nosema apis and Nosema ceranae are two typical types of them.They cause the Nosema disease.This article mainly introduces the distribution of the two Microsporidia spp.in China by cloning and sequencing of the segment of 16s rDNA.The results showed that Nosema ceranae was the agent of cause Nosema disease of Apis melliffera in most provinces of China.Nosema apis that was considered as the pathogen of Nosema disease in China has not been found until now.

关 键 词：蜜蜂微孢子虫 西方蜜蜂微孢子虫 东方蜜蜂微孢子虫 16s RRNA基因 

分 类 号：S895[农业科学—特种经济动物饲养] Q96[农业科学—畜牧兽医]