正常人外周血单核细胞基因表达谱的建立及临床意义  

Establishment and Clinical Significance of the Gene Expression Map of Normal Peripheral Blood Mononuclear Cells

作　　者：朱海霞[1] 翟宝进[1] 刘欣民[1] 崔刚[1] 董俊强[1] 王俊妨[1] 

机构地区：[1]武警医学院附属医院神经外科,天津300162

出　　处：《天津医药》2008年第11期856-858,共3页Tianjin Medical Journal

摘　　要：目的:建立正常人外周血单核细胞的基因表达谱,探讨颅内动脉瘤的差异基因,为研究颅内动脉瘤的发病机制及其可能的基因治疗打下基础。方法:分别抽提60例正常人和颅内动脉瘤患者外周血单核细胞的RNA,基因芯片技术检测其外周血中的基因表达情况。结果:在基因芯片所检测的共14112个基因中,有7749个基因在正常人外周血单核细胞中呈阳性表达,表达数量较多的基因分别是:细胞信号和传递蛋白表达类、代谢相关类、离子通道和运输蛋白、结构运动类基因。表达水平位于前3位的基因分别是核糖体蛋白L41(RPL41)、核糖体蛋白S3A(RPS3A)和核糖体蛋白L23A(RPL23A)基因。与正常人外周血阳性基因比较,颅内动脉瘤患者得到85个差异基因,其中KPNA4基因与信号传导、蛋白质转运过程功能有关,基因表达明显上调;SPG3A基因与核苷酸结合功能相关,基因表达明显下调。结论:建立了正常人外周血的基因表达谱,KPNA4基因和SPG3A基因可能与颅内动脉瘤形成和发展有关。Objective：To establish the gene expression map of normal peripheral blood mononuclear cells and to study the mechanism and the gene therapy of intracranial aneurysm（IA）.Methods：The total RNA was collected from 60 normal people and patients with IA.The gene chip technology was used to analyze the genetic expression of intracranial arterial blood.Results：The gene chip was used to detect 14 112 genes.There were 7 749 genes expressed in the normal peripheral blood.The gene expression levels were more in gene/protein expression categories,metabolism category and the structure/ movement genome.Of which,L41（RPL41）,S3A（RPS3A） and L23A（RPL23A） were the first third of the gene expression.Com-pared to IA patients,there were 85 differently expressed genes,the KPNA4 gene expression was up-regulated,which was relat-ed to signal conduction and protein transmission.The SPG3A gene expression was down-regulated,which was related to nu-cleotide conjunctin.Conclusion：The gene expression map of the normal peripheral blood was established.KPNA4 and SPG3A may be related to the development of IA.

关 键 词：单核细胞 基因表达 颅内动脉瘤 RNA 

分 类 号：R392.12[医药卫生—免疫学] R735.2[医药卫生—基础医学]