产ESBLs肺炎克雷伯菌中整合子和耐消毒剂基因的研究  

Integron and Antiseptic Genes in ESBLs-Producing Klebsiella Pneumoniae

作　　者：路秀文[1] 吕锦[1] 郭晓琳[1] 杨秀华[1] 张淑萍[1] 王金良[1] 

机构地区：[1]天津市公安医院检验科,300040

出　　处：《天津医药》2008年第11期866-869,共4页Tianjin Medical Journal

基　　金：天津市公安局科技基金资助项目(项目编号:04KY03)

摘　　要：目的:研究产超广谱β内酰胺酶(ESBLs)肺炎克雷伯菌整合子携带耐药基因状况及其对常用消毒剂的耐药性。方法:采用PCR方法检测77株产ESBLs和71株非产ESBLs肺炎克雷伯菌Ⅰ整合酶基因,分析Ⅰ类整合子与耐药性关系,并用耐消毒剂试验对耐消毒剂基因予以证实。结果:产ESBLs和非产ESBLs菌株中Ⅰ类整合酶扩增阳性率分别为66.2%(51/77株)和10.0%(2/20株),两者差异有统计学意义(P<0.05)。整合子阳性株对磺胺类、氨基糖苷类和环丙沙星的耐药率较高,与整合子阴性相比差异具有统计学意义(均P<0.01);耐消毒剂基因阳性株对常用消毒剂耐药能力高于阴性株。结论:Ⅰ类整合子在产ESBLs肺炎克雷伯菌中的分布明显高于非产酶株,并参与产ESBLs株多重耐药性的形成,携带耐消毒剂基因的菌株具有抗常用消毒剂的能力。Objective：To investigate the integron,drug-resistant genes and antiseptic genes in extended-spectrum be-ta-lactamases（ESBLs）-producing Klebsiella pneumoniae.Methods：The typeⅠintegron among 77 ESBLs-producers and 71 non-ESBLs-producers of Klebsiella pneumoniae were detected by PCR.The correlation between integron and drug-resis-tant genes were analyzed.It was validated for antiseptic genes by the test of disinfector.Results：The 66.2%（51/77） isolates were positive for classⅠintegron gene among ESBLs-producers,contrasted to 10.0%（2/20） in non-ESBLs-producers.The positive strains for classⅠintegron were related to multidrug resistance with high resistant rate to sulfonamides,aminoglyco-sides and ciprofloxacin（ P 〈 0.05 ）.There was higher drug-resistant ability in antiseptic gene-positive strains than those of antiseptic gene-negative strains.Conclusion：Distribution of class Ⅰintegron in ESBLs-producing strains was more predom-inant than that in non-ESBLs-producers.It contributed to multidrug resistance in ESBLs-producing strains.The strains car-rying antiseptic-resistance genes exhibited resistance to various antiseptics.

关 键 词：克雷伯菌 肺炎 整合子类 基因 药物耐受性 消毒药(剂) 

分 类 号：R378.21[医药卫生—病原生物学] R978.11[医药卫生—基础医学]