神经干细胞移植促进脊髓损伤后脑源性神经营养因子的表达  

Neural stem cells transplantation promote the expressions of brain derived neurotrophic factor after the spinal cord injury of rats

作　　者：王岩峰[1] 吕刚[2] 赵宇[1] 金哲[1] 黄涛[1] 于德水[1] 董宝铁[1] 

机构地区：[1]中国医科大学附属第一医院骨科,辽宁沈阳110001 [2]辽宁医学院

出　　处：《中国骨伤》2008年第11期836-838,共3页China Journal of Orthopaedics and Traumatology

基　　金：教育部高校博士点专项科研基金(编号:20060159019);辽宁省自然科学基金项目(编号:20052096)

摘　　要：目的:探讨神经干细胞(NSCs)移植对大鼠脊髓损伤后脑源性神经营养因子(BDNF)表达的影响及其意义。方法:NSCs提取自新生Wistar大鼠的海马区,经培养、鉴定。制作大鼠脊髓损伤(SCI)模型,于伤后第7天移植NSCs。实验分为3组:NSCs移植组(A组),DMEM填充组(B组),正常对照组(C组)。应用RT-PCR法和免疫组化法观察细胞移植后BDNF基因表达的变化。结果:RT-PCR结果分析,移植术后第1、3、5天,A组BDNFmRNA的表达量明显高于B组,差异有统计学意义(P<0.05)。组化结果分析,移植术后第7、14、28天BDNF的表达量A组明显高于B组,差异有统计学意义(P<0.05)。结论:NSCs在移植后可上调脑源性神经营养因子BDNF基因的表达,是其修复脊髓损伤的机制之一。Objective：To observe the effects of neural stem cells （NSCs） transplantation on the brain derived neurotrophic factor（BDNF） after the spinal cord injury（SCI） of rats, and to investigate the mechanism of repairing the SCI by NSCs transplantation. Methods：Neural stem cells were cultured from the hippocampus of rats＇ embryo and identified by immunocytochemistry. Seven days after the operation of SCI,the NSCs were transplanted into the injured site. Sixty adult Wistar rats were randomly divided into three groups ：SCI cured with NSCs transplantation （group A）, SCI received DMEM solution （group B）, control group（group C ）. Then the expression of BDNF of the lesion and neighbor areas were examined by reverse transcsription polymerase chain reaction （RT-PCR） and immunohistochemistry, so as to investigated the mechanism of repairing the SCI after NSCS transplantation. Results：According the RT-PCR results analysis,the expression of BDNF mRNA of group A enhanced higher than that of group B on the 1st, 3rd, 5th day after transplantation of NSCs. According the immunohistochemistry results analysis ,the expression of BDNF mRNA of group A enhanced higher than that of group B on the 7 th, 14 th, 28 th day similarly. Conclusion：The transplantation of NSCs can change the tiny-entiromnent by upregulating the expression of BDNF. It maybe one of the mechanism of repairing the SCI by NSCs transplantation.

关 键 词：脊髓损伤 神经干细胞 脑源性神经营养因子 

分 类 号：R651.2[医药卫生—外科学] R-332[医药卫生—临床医学]