阿胶强骨口服液对去卵巢骨质疏松大鼠骨密度、生物力学、25-(OH)D_3和1,25-(OH)_2D_3的影响  

Effects of Chinese kidney-tonifying drugs on bone mineral density(BMD),biomechanics,25-hydroxy Vitamin D_3 and 1,25-dihydroxy Vitamin D_3 of ovariectomized osteoporosis rats

作　　者：帅波[1] 沈霖[1] 杨艳萍[1] 谢晶[1] 周丕琪[1] 李恒[1] 郭向飞[1] 赵佳[1] 武嘉林 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,湖北武汉430022 [2]新疆华世丹药业股份有限公司

出　　处：《中国骨伤》2008年第11期850-853,共4页China Journal of Orthopaedics and Traumatology

基　　金：湖北省卫生厅资助课题(编号:鄂卫发2004-79)

摘　　要：目的:研究阿胶强骨口服液对去卵巢骨质疏松大鼠骨密度(BMD)、生物力学、25(OH)D3和1,25(OH)2D3的影响,探讨阿胶强骨口服液治疗原发性骨质疏松症的疗效机制。方法:4月龄健康雌性SD大鼠36只,随机分为3组,每组12只,分别为模型组,假手术组,阿胶强骨口服液治疗组。除假手术组外所有大鼠手术摘除双侧卵巢后导致雌激素缺失从而诱导骨质疏松症模型,分别在实验的第4、8、12周采用DEXA法分析股骨头及粗隆部的骨密度,生物力学技术分析股骨头生物力学参数,酶联免疫吸附方法检测25(OH)D3和1,25(OH)2D3的含量。结果:阿胶强骨口服液治疗组与模型组比较,股骨头及粗隆部骨密度明显提高(P<0.05);最大载荷(ML)及最大压应变(MS)等指标明显增强(P<0.05);血液、肝脏和肾脏组织中25(OH)D3和1,25(OH)2D3的含量明显提高,且组间比较差异有统计学意义(P<0.05)。阿胶强骨口服液治疗组与假手术组比较差异无统计学意义(P>0.05)。结论:阿胶强骨口服液在雌激素缺失早期即可在蛋白水平上调节25(OH)D3和1,25(OH)2D3的表达,激活骨代谢,提高骨密度,增强骨质量,起到预防骨质疏松的作用。Objective ：To investigate the effects of Chinese kidney-tonifying drugs on bone mineral density ,biomechanics, 25-hydroxy Vitamin D3 and 1,25-dihydroxy Vitamin D3 of ovariectomized osteoporosis rats, and explore the mechanism of treating osteoporosis with the drugs. Methods ：Thirty-six female SD rats （four months） were randomly divided into model group, sham group and treatment group. All the rats had been ovariectomied except those in sham group. Selecting 4,8,12 weeks in the experiment, the value of bone mineral density （BMD） was measure by dual energy X-ray absorptiometry （DEXA） of femoral head ,while the biomechanics machine was applied to analysis femoral head biomechanies index and ELISA method was used to detect the content of 25-hydroxy Vitamin D3 and 1,25-dihydroxy Vitamin D3 discern in blood-serum,liver and kidney. Resuits： Treatment group rats＇ BMD of femoral head was enhance compared with model group, significant differences were absent （P〈0.05）, and the maximal load and maximal stress measurement were improved, significant differences were absent （P〈0.05）. As the content of 25-hydroxy Vitamin D3 and 1,25-dihydroxy Vitamin D3 discern in blood-serum,liver and kidney were elevate, furthmore there were significant differences in group comparison, all significant differences were absent（P〈0.05 ）. But those compared with sham group, there was no significant difference （P〉0.05） Conclusion：In the early period in absence of estrogenic hormone,the Chinese kidney-tonifying drugs could activate bone metabolism to raise BMD and reinforce quality of bone through up-regulating expression of 25-hydroxy Vitamin D3 and 1,25-dihydroxy Vitamin D3 at protein level.

关 键 词：1 25-二羟基维生素D3 骨密度 骨生物力学 骨质疏松 骨代谢 

分 类 号：R563[医药卫生—呼吸系统] R274.91[医药卫生—内科学]