氯沙坦对自发性高血压大鼠肾脏蛋白质表达谱的影响  

LOSARTAN'S EFFECTS ON THE PROTEIN EXPRESSION OF THE KIDNEY OF SPONTANEOUS HYPERTENSION RAT

作　　者：杜于茜[1] 余敏[2] 陈君柱[2] 王兴祥[2] 孙坚[2] 李淑萍[2] 

机构地区：[1]浙江省台州医院心内科,浙江临海317000 [2]浙江大学附属第一医院,浙江杭州310003

出　　处：《中国应用生理学杂志》2008年第4期468-473,共6页Chinese Journal of Applied Physiology

基　　金：浙江省重点医学基金

摘　　要：目的:研究氯沙坦对自发性高血压大鼠肾脏组织蛋白质表达的影响。方法:采用蛋白质组学的技术,建立自发性高血压和氯沙坦干预后高血压大鼠肾脏的蛋白质二维凝胶电泳图谱,利用Imagemaster2Dv5.0软件分析蛋白点,并通过LC-MS/MS质谱分析和数据库检索鉴定差异蛋白质。结果:两组凝胶的平均蛋白点数分别为570±48、686±30。氯沙坦干预后有13个蛋白表达发生了显著变化,表达增强4个,表达降低4个,蛋白点消失5个。13个差异蛋白点进行质谱分析,鉴定出的7个蛋白质为Heat shock protein(Hsp)、Tubulin alpha-1chain、Transthyretin precursor、Liver regeneration-related protein LRRG03、Ezrin-radixin-moesin binding phosphoprotein50、Phosphoglycerate kinase1、Anionic trypsin I precursor。结论:这些差异表达的蛋白质可能在氯沙坦对高血压肾脏保护中发挥一定作用。Aim： To analyse the alterations of protein expression in the kidney of spontaneously hypertensive rat with losartan. Methods： The proteins of the kidney were isolated by two-dimensional gel electrophoresis. The protein spots with significant changes were selected for further identification by LC-MS/MS. Results： The number of average protein spots of two groups was 570 ±48 and 686 ±30 respectively. Compared with the SHR, 13 spots had changed significantly after treated with losartan. There were 5 protein spots detected only in SHR group, while 4 up-regulated and 4 down-regulated protein spots were detected in SHR-L group. These differentially expressed proteins were detected by mass spectrometry. 7 spots were identified. There were Heat shock protein（Hsp）, Tubulin alpha-1 chain, Transthyretin precursor, Liver regeneration-related protein LRRG03, Ezrin-radixin-moesin binding phcsphoprotein 50, Phosphoglycerate kinase 1 and Anionic trypsin I precursor. Conclusion： The different protein spots expression may play important roles in Losartan＇s effective protection to hypertension rats renal tissue.

关 键 词：肾脏 高血压 蛋白质组 氯沙坦 

分 类 号：R285.3[医药卫生—中药学]