骨髓间充质干细胞自体移植治疗大鼠脑冷冻伤的实验研究  

Curative Effect of Autologous Transplantation of Mesenchymal Stem Cells on the Cold-induced Brain Injury in Rats

作　　者：薛洪利[1] 刘洋[1] 刘民培[1] 冯新莉[1] 陈游力[1] 吕占举[1] 

机构地区：[1]沈阳军区总医院神经外科,沈阳110016

出　　处：《中国临床神经外科杂志》2008年第11期673-677,共5页Chinese Journal of Clinical Neurosurgery

摘　　要：目的探索骨髓间充质干细胞(MSCs)对脑损伤的治疗作用。方法将Wistar大鼠自体骨髓MSCs在体外扩增并经Brdu标记后,通过颈内动脉注射将其植入冷冻伤脑水肿动物体内,从组织化学和神经功能评分两个方面观察骨髓MSCs自体移植的治疗作用。实验动物分为4组;A组给予常规治疗;B组给予干细胞自体移植;C组在进行干细胞自体移植的同时给予神经节苷脂和丹参注射液治疗;D组在干细胞自体移植前给予罂粟碱开放血脑屏障。结果经颈内动脉注射的Brdu标记的大鼠自体骨髓MSCs细胞可向脑损伤区域迁移。B、C、D组Brdu阳性细胞的数量和神经功能恢复的程度均高于A组(P<0.05)。与B组相比,C组未增加Brdu阳性细胞的数量,但是神经功能恢复的程度明显升高(P<0.05)。D组Brdu阳性细胞的数量、神经功能恢复的程度均高于B组(P<0.05),但神经恢复的程度与C组相比无明显差异(P>0.05)。结论骨髓间充质干细胞可以通过循环系统向脑损伤区迁移而发挥其治疗作用。Objective To explore the curative effect of autologous transplantation of bone mesenchymal stem cells （MSCs） on severe brain injury induced by cold. Methods After MSCs from the Wistar rats were proliferated, MSCs were labelled with Brdu in vitro. The Brdu-marked autologous MSCs were injected into the internal carotid arteries in the rats with severe brain injury induced by cold. Eighty-four rats wered averagely divided four groups, i.e. group A, where the rats were routinely treated, group B, where the rats were treated by autologous transplantation of MSCs, group C, where the rats were treated by autologous transplantation of MSCs, and GM1 and salvia militiorrhiza injection, and group D, where the rats were treated by papaverine injection before MSCs were autologously transplanted. The curative effects in all the groups were observed. Results The Brdu-labelled MSCs were observable in the injured brain areas. The amount of Brdu positive cells in the injured brain area in groups B, C and D and the points of the neural function in group B were significantly more than those in the group A （P〈0.05）. There was insignificant difference in the amount of Brdn positive cells between group C and group B, but the points of the neural function were significantly higher in group C than those in group B 4 weeks after the autologous transplantation of MSCs. The amount of Brdu positive cells were significantly more in group D than that in groups B and C （P〈0.05）. The points of the neural function were significantly higher in group D than those in group B （P〈0.05）. Conclusion The autologous MSCs labelled with Brdu can immigrate into the injured brain area after the injection of the internal carotid artery and exert the curative effect in the rats with cold-induced severe brain injury.

关 键 词：脑损伤 间充质干细胞 自体移植 迁移 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R-332[医药卫生—基础医学]