原花青素对卵巢癌SKOV3细胞增殖和凋亡的影响  

Grape seed proanthocyanidin extract inhibits proliferation and promotes apoptosis of ovary cancer cell line SKOV3

作　　者：池余刚[1] 钟玲[1] 伍霞[1] 王勇[1] 

机构地区：[1]重庆医科大学附属第二医院妇产科,重庆400010

出　　处：《第三军医大学学报》2008年第23期2203-2206,共4页Journal of Third Military Medical University

摘　　要：目的探讨葡萄籽提取物原花青素对卵巢癌SKOV3细胞增殖和凋亡的影响和机制。方法体外培养的SKOV3细胞与25、50、100、200、400μg/ml的葡萄籽提取物原花青素共孵育,分别在24、48、72h时观察细胞形态学变化,MTT检测细胞增殖抑制率,流式细胞和TUNEL分析细胞凋亡,RT-PCR检测survivin在mRNA的表达情况,Western blot检测SKOV3细胞survivin在蛋白水平的表达。结果不同浓度(25、50、100、200、400μg/ml)原花青素对SKOV3细胞作用48h的细胞增殖抑制率分别为48.67%、53.85%、67.03%、73.78%、77.39%。流式细胞和TUNEL检测经25μg/ml原花青素处理24、48h后SKOV3细胞凋亡率分别为31.98%、45.78%。葡萄籽原花青素还可以明显降低survivin的mRNA和蛋白水平的表达。这些作用均随原花青素浓度和作用时间的延长而增强。结论原花青素可能通过降低survivin的表达,在体外抑制SKOV3细胞增殖,并促进其凋亡。Objective To study the biological effects of grape seed proanthocyanidin extract （GSPE） on human ovary cancer cells and to explore the molecular mechanism. Methods Human ovary cancer cell line SKOV3 were co-cultured with GSPE solution of the terminal concentrations of 25, 50, 100, 200, 400μg/ml respectively in 96-well plate. At 24, 48, 72 h after coculture, the following parameters were detected ： cell growth curve, the inhibition rate of SKOV3 cells by MTT assay, DNA cycle by FCM, the apoptosis of SKOV3 by TUNEL and Annexin-V labeling method, the mRNA and protein expressions of survivin by semi-quantitative RT-PCR and Western blotting, respectively. Results GSPE dose-dependently inhibited the proliferation of the SKOV3 cells. Treated by GSPE, the progress of SKOV3 cells at S stage into G/M stage was inhibited. TUNEL showed that treated by 25 μg/ml GSPE for 24, 48 h, the apoptotic rates of SKOV3 cells were 31.98%, 45.78% respectively. Annexin-V showed that after incubation with 25 μg/ml GSPE for 24 h, the apoptotic rate was 14.68%. The survivin mRNA and protein expressions were both down-regulated. Conclusion GSPE inhibits the proliferation of malignant human ovary cancer cells and induces their apoptosis. Expression of survivin mRNA and protein may be related to cell growth inhibition and to the apoptosis mediated by GSPE in vitro.

关 键 词：卵巢癌 原花青素 细胞增殖 细胞凋亡 SURVIVIN SKOV3细胞 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R73-361[医药卫生—基础医学]