尾加压素Ⅱ致体外培养乳鼠心肌细胞肥大效应研究  

Effect of urotensin Ⅱ on hypertrophy of cardiac myocytes from neonatal SD rats

作　　者：李巍[1] 李爱民[1] 郭瑞威[1] 郜攀[1] 

机构地区：[1]第三军医大学新桥医院心血管内科,全军心血管病研究所,重庆400037

出　　处：《第三军医大学学报》2008年第24期2293-2295,共3页Journal of Third Military Medical University

基　　金：重庆市自然科学基金(2007BB5026)~~

摘　　要：目的观察尾加压素Ⅱ(Urotensin Ⅱ,U Ⅱ)对心肌细胞肥大的效应。方法以体外培养的SD乳鼠心肌细胞为实验模型,分为正常对照组,单纯UⅡ作用组,环胞素A(CsA)阻断组。用real-time PCR方法分析β-MHC、CaN(钙调神经磷酸酶) mRNA表达的变化,用免疫印迹法(Western blot)研究心肌肥大过程中β-MHC、CaN蛋白表达的变化,探讨UⅡ对心肌细胞肥大的影响。结果①随着UⅡ浓度的增加,心肌细胞β-MHC、CaN mRNA和蛋白表达明显增加,其中10-8、10-7mol/L U Ⅱ组与对照组相比较差异显著(P<0.05);②用10-8mol/L U Ⅱ处理心肌细胞12、24、48 h,随着时间增加,心肌细胞β-MHC、CaN mRNA和蛋白表达呈递增趋势,其中处理24h组与正常对照组有显著差异(P<0.05);③预先用环胞素A5μmol/L(cyclosporin A CsA)处理正常心肌细胞24 h,然后用10-8mol/L U Ⅱ作用24 h,心肌细胞β-MHC、CaN mRNA和蛋白表达与正常心肌细胞经10-8mol/L U Ⅱ单纯作用24 h差异有显著意义(P<0.05)。结论 U Ⅱ能够诱导心肌细胞的肥大,环胞素A(CsA)能阻断此效应,CaN参与了UⅡ诱导的心肌肥大过程。Objective To explore the mechanism by which urotensin Ⅱ induces hypertrophy of the cultured rat cardiomyocytes. Methods The cultured cardiomyocytes from neonatal SD rats were treated with urotensin Ⅱ , also with cyclosporine A for its blocking effect on urotensin Ⅱ induced cardiomyocytes hypertrophy. The mRNA and protein levels of ^-MHC and CaN were evaluated by real-time PCR and Western blotting, respectively. Results In the cells treated with 10^-8 and 10^-7mol/L urotensin Ⅱ, the mRNA and protein levels of β-MHC and CaN were significantly higher than that of control （P 〈 0.05 ）. Treatment of cardiomyocytes with 10^-8 mol/L urotensin Ⅱ for 12 -48 h induced a time-dependent increase of β-MHC and CaN mRNA and protein expressions （P 〈 0.05）. Urotensin Ⅱ -induced upregulation of β-MHC and CaN mRNA and protein expressions can be abated by 24-hour cyclosporine A pretreatment （P 〈0.05）. Conclusion Urotensin Ⅱ can induce cardiomyocytes hypertrophy and eyclosporine A can block this effect. CaN participates in this process. Our results may contribute to the investigation of cardiaomyocyts hypertrophy induced by hypertension and other cardiac diseases.

关 键 词：尾加压素Ⅱ 心肌细胞 肥大 钙调神经磷酸酶 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]