胆道闭锁患儿肝组织细胞因子及相关基因表达的研究  

Cytokine expressions in biliary atresia

作　　者：张志波[1] 胡传兵[1] 高红[1] 王练英[1] 

机构地区：[1]中国医科大学盛京医院小儿外科,沈阳110004

出　　处：《中华小儿外科杂志》2008年第11期667-670,共4页Chinese Journal of Pediatric Surgery

摘　　要：目的研究胆道闭锁（BA）患儿肝脏组织中上皮-间充质转化过程中特异性细胞因子的表达及胚胎期发育相关基因的表达激活，旨在探讨BA肝脏纤维化等系列改变的分子机制。方法取经手术证实为BA患儿的8例肝活检组织（年龄2～3个月），以无消化道疾病尸检婴儿8例肝脏组织做正常对照（年龄042个月），RT-PCR半定量法检测胆管上皮细胞特异性因子CK19，纤维化早期指标I型胶原蛋白COL1A1，发育相关基因Notch基因受体HES1，TGF-β及其阳性信号分子Smad3的表达水平变化。结果BA患儿肝脏组织中CK-19、COL1A1、HES1、TGF-β及Smad3等基因表达水平均明显提高，实验组及对照组差异显著。结论BA患儿肝脏组织胶原组织增生，同时有胆管上皮细胞增生，胚胎期肝脏组织发育相关基因Notch信号通路系统重新激活，TGF-β及其阳性信号分子Smad3在这一过程中发挥作用。Objective To study the expression of special cytokines and related genes in patients with biliary atresia. Methods Liver biopsy specimen from 8 infants （age 1-3 months） with biliary atresia and liver tissue from 8 infants （age 1-3 months） without digestive disease were studied. RT- PCR of epithelium specific eytokine CK19, early fibrosis factor COLAI, developmental gene Notch receptor HES1, TGF-β and positive receptor Smad3 were performed. Results The expressions of CK- 19, COLA1, HES1, TGF-β, Smad3 were all up-regulated in liver tissues from biliary atresia patients. The differences were significant. Conclusions In livers of biliary atresia, there＇s proliferation of bile duct epithelium. The collagen gene CLA1, developmental gene Notch, TGF-β and its transducer, Smad3 were up-regulated. This suggests a possible role for these genes.

关 键 词：胆道闭锁 肝纤维化 细胞因子类 

分 类 号：R686[医药卫生—骨科学]