大黄素等中药成分抗肿瘤-血管内皮细胞黏附作用及机制研究  

Anti-Adhesion Effect of Emodin on Tumor Endothelial Cells and Its Mechanism

作　　者：曾雄义[1] 可燕[1] 朱春赟 

机构地区：[1]上海中医药大学教学实验中心,上海201203

出　　处：《上海中医药大学学报》2008年第6期50-53,共4页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市教委科研基金资助项目(05JG05053)

摘　　要：目的:考察活血化瘀中药成分抗肿瘤-血管内皮细胞黏附能力及其机制。方法:运用体外荧光标记肿瘤-内皮细胞黏附模型对中药成分抗肿瘤细胞黏附能力进行考察,并运用Western blotting实验手段检测相关黏附分子蛋白表达。结果:对几种活血化瘀中药成分进行筛选后发现大黄素具有一定的抗肿瘤-内皮黏附能力,同时可降低内皮细胞黏附分子ICAM-1及E-selectin的表达。结论:大黄素具有抗肿瘤-内皮细胞黏附能力,可能是通过降低黏附分子表达的机制实现的。Objective： To investigate the anti-adhesion effect of Chinese medicine extracts effective in activating blood and removing stasis on tumor endothelial cells and its mechanism. Methods： The model was established with the MDA-MB-231 breast cancer cells and monolayers of human umbilical vein endothelial cells （HUVEC） in vitro. The tumor cells were specifically labeled with a fluorescent dye for quantification. For increasing the amount of adherent cells, HUVEC monolayers were stimulated with phorbol ester（TPA） before the addition of the tumor ceils. To investigate the possi ble mechanism of Emodin＇s anti-adhesion tumor cells to endothelial cells, the expression of adhesion molecules ICAM-1, VCAM-1, E-selectin were studyed with Western blot assay. Results： Among several extracts tested, Emodin reduced tumor cell＇s adhesion to HUVECs to 89.50 % at 1μg/mL, 78.13 % at 2 μg/mL and 77.12 % at 6μg/mL, and the expression of CAM-1 and E-selectin. Conclusion： Emodin has the effect of reduceing tumor cell＇s adhesion to HUVECs by down-regulating the expression of adhesion molecules.

关 键 词：肿瘤转移 肿瘤细胞黏附 原代人脐静脉内皮细胞 黏附分子 大黄素 

分 类 号：R285.5[医药卫生—中药学]