PLFAs稳定同位素技术及其在土壤微生物学中的应用  

Stable Isotope Techniques of PLFAs and It’s Application in Soil Microbiology

作　　者：宋斗妍[1,2] 白震[1,2] 何红波[1] 张旭东[1,3] 解宏图[1,3] 郑立臣[1,3] 

机构地区：[1]中国科学院沈阳应用生态研究所陆地生态过程重点实验室,辽宁沈阳110016 [2]中国科学院研究生院,北京100039 [3]辽宁沈阳农田生态系统国家野外研究站,辽宁沈阳110016

出　　处：《土壤通报》2008年第6期1475-1479,共5页Chinese Journal of Soil Science

摘　　要：磷脂脂肪酸(PLFAs)是微生物细胞膜的重要组分,可作为活体微生物的生物标记物。稳定同位素技术与生物标记物相结合为揭示微生物种群结构及其功能提供了一种有效的方法,可用来阐明复杂土壤生态系统中微生物源有机质代谢途径以及个别微生物种群特征,将特定微生物种群与相应生物化学过程相联系。介绍了PLFAs稳定同位素分析技术,包括气相色谱-燃烧-同位素比例质谱(GC-c-IRMS),气相色谱-质谱联机(GC-MS)以及核磁共振(NMR),并描述上述方法在土壤微生物学中的应用以及其优缺点。PLFAs are important components of cell membrane and can be used as biomarkers of active microorganisms. The application of stable isotope techniques in PLFAs＇ analysis could be an effective way to elucidate the community structures and functions of soil microbiology. These techniques are used to illuminate the metabolic pathway of microbial organic matter, identify the characteristics of particular microbial communities and link directly microbial populations with the corresponding biochemistry process. In this review, three different stable isotope techniques of PLFAs analysis had been introduced, including GC-c-IRMS （gas chromatography combustion isotope ratio mass spectrometry）, GC-MS （gas chromatography mass spectrometry） and NMR （nuclear magnetic resonance） techniques. These techniques＇ applications in soil microbiology were also introduced.

关 键 词：磷脂脂肪酸 稳定同位素 土壤微生物 

分 类 号：S154.36[农业科学—土壤学] S152[农业科学—农业基础科学]