耐药性颞叶癫痫脑组织中UL16结合蛋白2(ULBP2蛋白)的表达及临床意义  

The overexpression of ULBP2 in the brain of drug-refractory epilepsy

作　　者：关立峰[1] 王学峰[1] 林涛[1] 曾艳[1] 李劲梅[1] 肖飞[1] 孙纪军[1] 朱曦[1] 杨辉[2] 栾国明[3] 王玉平[4] 卢涌 李红卫 

机构地区：[1]重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室,重庆400016 [2]中国人民解放军第三军医大学附属新桥医院神经外科,重庆400037 [3]首都医科大学天坛医院神经外科,北京100062 [4]首都医科大学宣武医院神经内科,北京100052 [5]重庆刑事科学研究所,重庆400015

出　　处：《中国免疫学杂志》2008年第11期1003-1006,共4页Chinese Journal of Immunology

摘　　要：目的:研究耐药性颞叶癫痫患者脑组织中ULBP2的表达,探讨其在耐药性颞叶癫痫发病中的意义。方法:从重庆医科大学附属第一医院神经内科建立的耐药性癫痫患者脑组织库中随机抽取42例耐药性颞叶癫痫患者术后脑组织,用基因芯片检测ULBP2 cDNA的表达,用免疫荧光、免疫印记法(Western blot)检测ULBP2的蛋白表达产物,并与12例对照组进行比较。结果:耐药性颞叶癫痫患者颞叶脑组织中编码ULBP2的基因出现高表达;ULBP2蛋白在耐药性颞叶癫痫患者颞叶脑组织中的表达量与对照组相同部位比较明显增高。结论:耐药性颞叶癫痫患者颞叶脑组织中ULBP2基因及蛋白产物表达增高可能与耐药性颞叶癫痫发病中的免疫异常机制有重要关联,甚至可能是关键因素。它很可能为耐药性颞叶癫痫的免疫治疗提供新的靶点。Objective：To study the expression of ULBP2 protein in the brain tissues of patients with drug-refractory epilepsy and its clinical significance. Methods： Gene-chip, immunofiuorescence and Western blot were used to test expression of ULBP2 in the surgically removed brain tissue of patients with drug-refractory epilepsy from the brain bank of our department（ n = 42）, and the results were compared with that of normal controls （ n = 12）. Results： The relative increasing expression of ULBP2-gene in the brain of patients with drug-refractory epilepsy,and ULBP2 protein expression was significantly increased in temporal lobe cortex of patients with drug-refractory epilepsy as compared with the same regions of the controls specimens. Conclusion：The results indicate that the overexpression of ULBP2 may be involved in the patho- physiology of drug-refractory epilepsy.

关 键 词：ULBP2蛋白 癫痫 免疫反应 颞叶 

分 类 号：R392[医药卫生—免疫学]