小鼠口服青蒿琥酯纳米乳的急性毒性研究  

Study on the Acute Toxicity of Artesunate Nano-emulsion in Mice by Oral Administration

作　　者：胡宏伟[1,2] 周绪正[2] 李剑勇[2] 张继瑜[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050

出　　处：《安徽农业科学》2008年第31期13648-13649,13652,共3页Journal of Anhui Agricultural Sciences

基　　金：国家高技术研究发展计划(863计划)重大课题(2006A-A10A203);中央级公益性科研院所基本科研业务费专项资金项目(BRF070405)

摘　　要：[目的]研究3种不同配方青蒿琥酯（Artesunate,AS）纳米乳的急性毒性,筛选出安全性最好的配方。[方法]采用小鼠灌胃给药,观察14 d内小鼠的活动情况及死亡情况,记录小鼠的死亡数。用改良寇氏法测定各配方AS纳米乳的半数致死量（LD50）及可信限。[结果]AS1、AS2纳米乳的LD50分别为7.76和7.56 g/kg;可信限为6.90～8.68和6.42～8.87。AS3纳米乳的LD0为15 g/kg。[结论]在国内首次成功制备的3种不同配方AS纳米乳,均属于实际无毒范围,AS3纳米乳较之AS1、AS2更安全。[Objective] The research aimed to study the acute toxicity of 3 kinds of artesunate with different formula and screen out the formula with the best safety.[Method] The drugs were infused into stomach of mice to observe the activity situation and death situation of mice within 14 days.The number of dead mice was recorded.The median lethal dose（LD50） and confidence limit of AS nano-emulsion with different formula were determined by using improved Karber method.[Result] LD50 of AS1 and AS2 nano-emulsion were 7.76 and 7.56 g/kg resp.and the confidence limit of the two were 6.90-8.68 and 6.42-8.87 resp.LD50 of AS3 nanoemulsion was 15 g/kg.[Conclusion] 3 kinds of nano-emulsion with different formula prepared successfully in China for the time all had no actual toxicity.AS3 nano-emulsion was safer than AS1 and AS2.

关 键 词：小白鼠 青蒿琥酯纳米乳 半数致死量 急性毒性 

分 类 号：S865.13[农业科学—野生动物驯养]