倍他米松对毛囊外毛根鞘无色素黑素细胞激活的试验研究  

Betamethasone actives cultured amelanotic melanocytes derived from outer root sheath of human hair follicle

作　　者：王大光[1] 朱文元[1] 马慧军[1] 岳学状[1] 李诚让[1] 

机构地区：[1]南京医科大学第一附属医院皮肤性病科,江苏南京210029

出　　处：《临床皮肤科杂志》2008年第12期769-771,共3页Journal of Clinical Dermatology

基　　金：国家自然科学基金资助项目(30170861)

摘　　要：目的:研究倍他米松(betamethasone,BT)对毛囊无色素黑素细胞(amelanotic melanocytes,AMMC)的激活作用。方法:以高、中、低3种不同浓度的BT作用于培养的人毛囊外毛根鞘AMMC,测定药物作用前后酪氨酸酶(tyrosinase,TYR)活性和黑素生成的变化。通过间接免疫荧光法结合激光共聚焦显微镜半定量分析药物作用前、后AMMC TYR、酪氨酸酶相关蛋白(ty-rosinase related protein,TRP)-1和TRP-2表达的变化,透射电镜分析黑素小体在药物作用前后的变化。结果:BT促进了AMMC表达TYR和TRP-1,并且以剂量依赖方式促进TYR活性,诱导黑素生成。AMMC主要含有Ⅰ、Ⅱ和Ⅲ期黑素小体,但是BT作用后AMMC含有大量Ⅲ或Ⅳ期黑素小体,并且以Ⅳ期黑素小体为主。结论:BT能够激活AMMC,这可能部分解释了糖皮质激素治疗白癜风的机制。Objective： To investigate the effects of betamethasone（BT） on the differentiation and proliferation of amelanotic melanocytes （AMMC） derived from human hair follicles. Methods： The method using dispase was employed to culture AMMC of human hair follicles. Three concentrations（higer, middle and lower） of BT were incubated with AMMC. Before and after that, the tyrosinase activity and melanin content in AMMC after stimulation were tested respectively. Laser confocal scanning microscopy （LCSM） was employed to carry out semi-quantitive analysis of expression of TYR, TRP-1 and TRP-2 after staining of indirect immunofluorescence. The change of melanosomes were observes by transmission electron microscopy （TEM）. Results： BT increased expression of TYR and TRP-1, but not TRP-2. The tyrosinase activity and melanin contents of AMMC could be induced by BT in concentration-dependent manner. There were lots of stage Ⅰ , Ⅱ and Ⅲ melanosomes in AMMC, but all melanosomes were of the stage Ⅲ and majority of the stage Ⅳ through incubation with BT. Conclusions： The AMMC derived from the out root sheath of human hair follicle can be activated by BT. It can be deduced that effective treatment of vitiligo with glucocorticoid is associated activation and proliferation of AMMC.

关 键 词：毛囊 黑素细胞 无色素 倍他米松 酪氨酸酶 酶氨酸酶相关蛋白 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] Q257[医药卫生—基础医学]