化学杀雄剂EXP对油菜膜脂过氧化及保护酶活性的影响  

Effect of Chemical Gametocide EXP on Membrane Lipid Peroxidation and Protective Enzyme Activity in Rapeseed (Brassica napus)

作　　者：胡文智[1] 程科[1] 严静[1] 李昌珍[1] 于澄宇[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《安徽农业科学》2008年第32期13949-13950,共2页Journal of Anhui Agricultural Sciences

基　　金：西北农林科技大学"国家生命科学与技术培养基地"科研专项资金[2006-(1)-061]

摘　　要：[目的]为探讨油菜化学杀雄作用特征与机理,为新型化学杀雄剂的开发利用提供理论依据。[方法]对化学杀雄剂EXP诱导甘蓝型油菜品种秦油3号、L89雄性不育过程中叶片和花蕾中过氧化物酶、过氧化氢酶活性以及过氧化氢、丙二醛含量进行了测定。[结果]EXP处理后的油菜体内保护酶活性及过氧化氢、丙二醛含量发生显著变化,说明活性氧代谢出现了异常。同时,不同品种及器官对EXP处理的反应程度表现出较大差异。[结论]活性氧代谢失调与化学杀雄剂EXP诱导雄性败育具有关联性。[ Objective ] The aim of this study was to investigate the characteristics and mechanism of chemical emasculation in rapeseed and to provide the theoretical basis for development and utilization of new chemical gametocides. [ Method] The activity of peroxidase, catalase and the content of hydrogen peroxide, malondialdehyde in leaves and flower buds of Brassica napus cultivars Qinyou No. 3 and L89 induced by the chemi- cal gametocide EXP in the course of male sterility were studied. [ Result] Protective enzyme activity and the content of hydrogen peroxide, ma-londialdehyde in rapeseed treated with EXP changed significantly, which indicated that active oxygen metabolism was abnormal. Furthermore, there was a significant difference in the reaction degree of different cultivars and organs treated by EXP. [ Conclusion ] There was a correlation between the disturbance of active oxwen metabolism and the male sterility induced by chemical gametocide EXP.

关 键 词：甘蓝型油菜 化学杀雄剂 过氧化物酶 过氧化氢酶 过氧化氢 丙二醛 

分 类 号：S565.4[农业科学—作物学]