HrpZ激发对几种植物防御相关酶活性的影响  

Effect of HrpZ Excitation on Activities of Defense-related Enzymes in Several plants

作　　者：袁瑞玲[1,2] 孟小林[1] 徐进平[1] 王健[1] 鲁伟[1] 

机构地区：[1]武汉大学生命科学学院国家病毒学重点实验室,湖北武汉430072 [2]云南省林业科学院,云南昆明650204

出　　处：《安徽农业科学》2008年第32期13951-13953,14066,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]测定HrpZ激发对黄瓜、番茄和茄子3种防御酶活性的影响情况。[方法]以盆栽黄瓜、番茄和茄子幼苗为材料,研究了15μg/ml的HrpZ激发黄瓜、番茄和茄子3种植株幼叶后,不同时间苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)3种防御酶活性的影响变化。[结果]经HrpZ激发后,植株叶片中的3种防御酶活性均有不同程度的提高。HrpZ喷施黄瓜12、24和48 h后,PPO、PAL、POD活性相应比清水对照提高83.0%、54.1%和39.3%;HrpZ喷施番茄12、24和48 h后,POD、PPO、PAL活性相应比清水对照提高31.8%、44.6%和33.3%;HrpZ喷施茄子1和3 h,POD、PPO活性相应比清水对照提高228.4%和142.5%。[结论]HrpZ能诱导植物PAL、POD、PPO的表达,从而诱导植物防御反应和系统获得抗性,增强植物的抗病性。[Objective ] The aim was to detect the effect of HrpZ excitation on the activities of 3 defense enzymes ot Cucumis sativus, Lycoperstcon esculentum and Solarium melongena. [Method] With the seedlings of C. sativus, L. esculentum and S. melongena in pot as tested materials, the activity changes of 3 defense enzymes of phenylalanine ammonia lyase （PAL）, peroxidase （POD）, polyphenoloxidase （PPO） in different time were studied after the young leaves of the 3 plants of C. sativus, L. esculentum and S. melongena were excited by 15 μg/ml HrpZ. [ Result] The activities of the 3 defense enzymes in the plant leaves were all increased to a different degree after the plant leaves were excited by HrpZ. The activities of PPO, PAL and POD in C. sativus spayed by HrpZ for 12, 24 and 48 h resp. were 83.0%, 54.1% and 39.3% higher than that treated with water CK; the activities of POD, PPO and PAL in L. esculentum spayed by HrpZ for 12, 24 and 48 h resp. were 31.8% , 44.6% and 33.3% higher than that treated with water CK; the activities of POD and PPO in S. melongena spayed by HrpZ for 1 and 3 h resp. were 228.4% and 142.5% higher than that treated with water CK. [ Conclusion] HrpZ could induce the plant defense response and system to acquire resistance and enhance the resistance of plants by inducing the expression of PAL, POD and PPO in plants.

关 键 词：HrpZ 苯丙氨酸解氨酶 过氧化物酶 多酚氧化酶 诱导抗性 

分 类 号：S312[农业科学—作物栽培与耕作技术]