小球藻的分离及其DNA提取方法的研究  

Isolation of Chlorella vulgaris and Its DNA Extraction Methods

作　　者：任雪艳[1] 孔庆军[1] 王恒强[1] 占东霞[1] 张海黎[1] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003

出　　处：《安徽农业科学》2008年第32期13970-13971,共2页Journal of Anhui Agricultural Sciences

基　　金：石河子大学高层次人才启动基金(07002-500002061401)

摘　　要：[目的]从湖水中分离小球藻并提取其DNA。[方法]采用稀释法及水滴法分离小球藻，优化小球藻培养条件，并用改良CTAB法及SDS法提取其DNA。[结果]温度20～25℃、光照4．39～5．86W／m^2及转速100～150r／min的条件适于小球藻的培养；改良CTAB法适于提取小球藻DNA。[结论]该研究将有助于在分子水平研究小球藻，促进小球藻的开发和利用。[Objective]The aim of this study is to isolate Chlorella vulgaris （chlorella） and extract its genomic DNA. [Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples; the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method. [Result] The proper conditions for chlorella culture were as following： temperature 20 -25℃, illumination 4.39-5.86 W/m^2 and rotational speed 100-150 r/min; improved CTAB method was suitable for extracting genomic DNA from chlorella. [ Conclusion] The result is helpful for studying the chlorella at molecular level and promote the exploitation and utilization of chlorella resources.

关 键 词：小球藻 分离方法 DNA提取 改良CTAB法 

分 类 号：Q949.2[生物学—植物学]