马桑内酯对培养的大鼠海马胶质细胞的影响──胶质细胞增生及胶质原纤维酸性蛋白免疫细胞化学观察  

Effect of Coriaria Lactone on Cultured Rat Hippocampal Neuroglia──Gliosis and GFAP Immunocytochemical Observation

作　　者：周洁萍[1] 刘云华[1] 余达经[1] 朱长庚[1] 

机构地区：[1]同济医科大学基础医学院解剖学教研室,武汉430030

出　　处：《同济医科大学学报》1997年第1期1-3,T077,共4页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金!39330210

摘　　要：采用培养1周的单层海马细胞，培养基中加入2.5×10(-5)mol／L终浓度的马桑内酯（CoriariaLactone，CL）作用48h后，发现马桑内酯组细胞数目较对照组（不含CL）明显增加（P＜0.01），经胶质原纤维酸性蛋白（glialfibrillaryacidicProtein，GFAP）免疫细胞化学染色后发现，马桑内酯组GFAP样免疫反应明显增强，阳性细胞增多，突起及胶质丝较多，且相互交织成网。The one-week-cultured cells from the rat hippocampus were divided into two groups, experimental and control. In experimental group, medium contained 2. 5 ×10-5 mol/L coriaria lactone (CL). After culturing for 48 hours, the number of cel1s was counted and the glial fibrillary acidic protein-immunoreaction (GFAP-IR) of cells was detected by immunocytochemistry. The results showed that the number of cells was significantly increased in the experimental group (P＜0.01) compared to the controlled group, cells had the GFAP-JR strengthened markedly, the number of the GFAP-IR (+) cells also increased and the increased glial fibers interwove with each other to form a net.

关 键 词：马桑内酯 海马 神经胶质原 纤维酸性蛋白 细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] Q593.2[医药卫生—基础医学]