人胃黏膜蛋白质组双向电泳图谱的获得和分析  

Establishment and analysis of two-dimensional electrophoresis profiles for human gastric mucosa proteome

作　　者：刘国红[1] 孙芸[1] 呼林[1] 张钦宪[1] 

机构地区：[1]郑州大学基础医学院组织学与胚胎学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2008年第6期1198-1200,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:建立和优化人胃黏膜组织蛋白质双向凝胶电泳技术,获得高分辨率和重复性的人胃黏膜双向电泳图谱。方法:采用双向电泳技术分离人正常胃黏膜组织总蛋白,并对其关键环节和因素,如样品处理、上样量、电泳参数、染色方法等进行一系列的优化,凝胶染色后扫描图谱,Imagemaster 6.0软件比较分析。结果:获取的人胃黏膜双向电泳图谱蛋白质斑点清晰、重复性好,主要分布在等电点pI 4～7、相对分子质量20 000～90 000范围内。结论:成功建立了人胃黏膜组织蛋白双向电泳技术,获得了分辨率和重复性较好的人胃黏膜双向电泳图谱。Aim： To establish and optimize the two-dimensional gel electrophoresis（2-DE） technology for human gastric mucosa proteome,and obtain satisfied 2-DE map of human gastric mucosa proteome.Methods：A series of important factors,such as sample preparation,protein quantities,electrophoresis parameters,staining methods,were optimized to improve the resolution and repeatability.2-DE maps were analyzed after staining.Results： Higher resolution and repeatability 2-DE maps were obtained（pI 4-7,relative molecular mass 20 000-90 000）.Conclusion：The 2-DE technology for human gastric mucosa proteome has been established successfully.

关 键 词：蛋白质组学 双向电泳 胃黏膜 

分 类 号：R329.44[医药卫生—人体解剖和组织胚胎学]