血管内皮生长因子抗体对子宫内膜异位症小鼠皮下模型作用的实验研究  

Effect monoclonal antibody against vascular endothelial growth factor on the transplanted endometriosis lesions in SCID mice

作　　者：蒋红清[1] 刘亚杰[1] 李亚里[2] 

机构地区：[1]北京海淀区妇幼保健院,100080 [2]北京中国人民解放军总医院妇产科

出　　处：《中国妇产科临床杂志》2008年第6期453-456,I0001,I0002,共6页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：全军十五重点课题基金资助项目(01Z032)

摘　　要：目的研究血管内皮生长因子(vascular endothelial growth factor,VEGF)单克隆抗体对子宫内膜异位症(EMs)病灶生长行为及其血管生成的影响,为从抗血管途径治疗EMs提供依据。方法将EMs患者在位子宫内膜种植于SCID小鼠腹壁皮下,建立EMs鼠模型。接种第3周治疗组(n=10)腹腔注射VEGF单克隆抗体3 mg/kg/d,对照组(n=10)腹腔注射等体积PBS,连用14 d;测量病灶体积及组织称重,并采用免疫组化法测定微血管密度(microvessel density,MVD)和VEGF的表达。结果治疗组病灶重量及体积低于对照组(P<0.05),光镜下可见治疗组腺体萎缩,间质伴有不同程度的坏死;治疗组MVD及VEGF表达显著低于对照组(P<0.05)。结论VEGF单克隆抗体对EMs异位病灶的生长和血管生成有明显抑制作用,证实抗血管生成治疗EMs的策略具有可行性。Objective To investigate the effects of monoclonal antibody against vascular endothelial growth factor（VEGF） on the growth and angiogenesis of transplanted endometriosis lesions in the severe combined immunodeficiency disease（SCID） mouse model.Methods Eutopic endometrium from women with endometriosis was transplanted to SCID mice,and the mice were randomly divided into test group and control group.After two weeks of transplantation,the test group（n=10） was injected with VEGF monoclonal antibody（3 mg.kg-1.d-1） into the peritoneal cavity.The control group（n=10） was given same volume of sterile PBS（200 μl.d-1）.The size of endometriotic lesions in SCID mice was measured every three days,and all the treatment lasted for 14 days.Microvessel density（MVD） and the expression of VEGF were determined immunohistochemically.Results VEGF monoclonal antibody inhibited the growth of endometriotic lesions in mice（P〈0.05）.Microscopic examination showed the bulk of the endometrial tissue decreased and glands depauperated.The glandular epithelium partially degenerated,and necrotic debris was presented in the endometrial stroma.MVD and the expression of VEGF were decreased significantly in the test groups compared to the control group（P〈0.05）.Conclusions Monoclonal antibody against VEGF can effectively interfere the growth of endometriosis by inhibiting angiogenesis.This suggests that the use of angiostatic agents may be promising as a therapy for endometriosis.

关 键 词：子宫内膜异位症 VEGF单克隆抗体 SCID小鼠 

分 类 号：R711.71[医药卫生—妇产科学]