乙型肝炎病毒前S1蛋白在血清学诊断乙肝病毒复制中的研究  

Study of hepatitis B virus pre-S1 in diagnosing viral replication in chronic hepatitis patients

作　　者：李果[1] 高兵[1] 孙自镛[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院检验科,武汉430030

出　　处：《临床血液学杂志（输血与检验）》2008年第6期627-629,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:探讨前S1蛋白(Pre-S1)在血清学诊断乙肝病毒复制中的意义。方法:选择慢性乙型肝炎患者血清250例。分别测定每份患者血清中HBV标志物、前S1蛋白和HBV DNA。结果:HBeAg(+)组中前S1蛋白阳性率97.2%(70/72),HBV DNA阳性率100%(72/72)。HBeAg(-)组中前S1蛋白阳性率60.1%(107/178),HBV DNA阳性率63.5%(113/178)。前S1蛋白和HBV DNA的总符合率为89.6%(224/250),2者差异无统计学意义(P>0.05)。HBeAg和HBV DNA的总符合率为54.8%(137/250),2者差异有统计学意义(P<0.01)。HBsAg(-)中检出前S1蛋白阳性2例。结论:前S1蛋白比HBeAg更敏感地反映HBV复制情况,且可弥补由于HBsAg基因编码区突变造成的漏诊。Objective：To study the clinical significance of HBV Pre-S1 in diagnosing viral replication in chronic hepatitis patients. Method： 250 chronic hepatitis patients were selected randomly. Serum Pre-S1 and HBV markers were determined by enzyme immunoassay （EIA） and HBV DNA was tested by polymerase chain reaction （PCR）. Result：The positive rates of Pre-S1 and HBV DNA in the patients with HBeAg （＋） were 97. 2% and 100% ,respectively. The positive rates of Pre-S1 and HBV DNA in the patients with HBeAg （-） were 60.1% and 63.5 ,respectively. The total coincidences of Pre-S1 and HBeAg with HBV DNA were 89.6% and 54.8%, respectively. One Pre-S1 （＋） was found in 38 HBsAg （-） samples. Conelusion：Pre-S1 was more sensitive than HBeAg in diagnosing viral replication, and it could be used as a proper supplement when HBsAg genetic mutation occurred.

关 键 词：前S1蛋白 乙肝病毒DNA 乙肝标志物 

分 类 号：R521.6[医药卫生—内科学]