一氧化氮对慢性缺氧大鼠肺动脉内皮素基因表达的影响  

Impact of nitric oxide on endothelin gene expression in intrapulmoary ateries of chronic hypoxic rats

作　　者：杜军保[1,2] 李万镇[1,2] 赵斌 黎文[1,2] 

机构地区：[1]北京医科大学第一医院儿科 [2]北京积水潭医院

出　　处：《中华医学杂志》1997年第4期263-265,T004,共4页National Medical Journal of China

基　　金：国家自然科学基金

摘　　要：目的探讨一氧化氮对慢性缺氧大鼠肺动脉内皮细胞及平滑肌细胞内皮素－１（ＥＴ－１）ｍＲＮＡ表达的影响，全面了解缺氧性肺动脉高压的发病机制。方法对经Ｌ－精氨酸（Ｌ－Ａｒｇ）及Ｎω－硝基－Ｌ－精氨酸甲酯（Ｌ－ＮＡＭＥ）处理的慢性缺氧大鼠（４０只）肺组织，以ＥＴ－１ｃＲＮＡ探针进行原位杂交。结果缺氧１周及缺氧２周大鼠大部分肺动脉（７５％±３％及７１％±６％）其１％～５０％的内皮细胞显示ＥＴ－１ｍＲＮＡ杂交阳性信号。Ｌ－Ａｒｇ使大鼠肺动脉内皮细胞ＥＴ－１ｍＲＮＡ表达明显抑制，而Ｌ－ＮＡＭＥ使其表达明显增强。缺氧１周及缺氧２周大鼠大部分肺动脉（８５％±６％及９８％±２％）的平滑肌细胞无ＥＴ－１ｍＲＮＡ表达，但Ｌ－ＮＡＭＥ使其表达增强。结论一氧化氮对慢性缺氧大鼠肺动脉内皮细胞及平滑肌细胞ＥＴ－１－ｍＲＮＡ表达均有抑制作用。Objective\ To investigate the impact of nitric oxide on endothelin 1(ET 1)mRNA expression in pulmonary artery endothelial cells and pulmonary artery smooth muscle cells of chronic hypoxic rats.Methods\ In situ hybridization was performed on lung sections from40chronic hypoxic rats treated either with L Arginine(L Arg)or N ω nitro L arginine methyl ester(L NAME)by using cRNA probe for ET 1. Rresults\ Most intrapulmonary arteries had1%～50%of the endothelial cells expressing ET1mRNA in both one week and two week hypoxic rats(75%±3%and71%±6%,respectively), which was significantly inhibited by L Arg but augmented by L NAME administration.Most pulmonary artery smooth muscle cells showed no ET 1mRNA signals in both one week and two week hypoxic rats(85%±6%and98%±2%,respectively).However,L NAME increased ET 1mRNA expression in pulmonary artery smooth muscle cells of hypoxic rats.Conclusion Nitric oxide Inhibited ET gene expression in both pulmonary artery endothelial cells and smooth muscle cells of rats exposed to chronic hypoxia.

关 键 词：一氧化氮 肺动脉 内皮素1 基因 表达 

分 类 号：R543.203[医药卫生—心血管疾病] R361.3[医药卫生—内科学]