模拟失重大鼠肝组织中NF-κB的表达及意义  

Expression of nuclear factor-kappa B and its significance in liver tissues of rats during simulated weightlessness

作　　者：崔彦[1,2] 董家鸿[1] 张铭[3] 周金莲[3] 刘子沛[2] 王平[2] 李成林[2] 张建中[3] 

机构地区：[1]中国人民解放军第三军医大学西南医院全军肝胆外科研究所,重庆市400038 [2]中国人民解放军第306医院普通外科,北京市100101 [3]中国人民解放军第306医院病理科,北京市100101

出　　处：《世界华人消化杂志》2008年第31期3480-3484,共5页World Chinese Journal of Digestology

基　　金：全军"11.5"计划面上基金资助项目;No.06MA005~~

摘　　要：目的:研究模拟失重环境下大鼠肝脏组织中NF-κB的表达及意义.方法:成年♂Wistar大鼠84只,随机分为模拟失重组和对照组,每组又分别设1、2、3、4、5、6和7d共7个时相点,每时相点模拟失重和同步对照各6只大鼠.采用尾悬吊法建立模拟失重动物模型.各组大鼠肝组织中NF-κB表达分别应用Western blot和免疫组化PV-6001法进行检测.结果:在尾悬吊1-2d期间,大鼠焦躁不安,饮食量减少,精神较差,活动减弱,2-3d后有所适应,逐渐恢复稳定状态.模拟失重环境下大鼠肝脏NF-κB表达水平明显升高,1、2d悬尾组大鼠肝组织中NF-κB表达率明显高于对照组(F=271.36,P<0.01),其后,NF-κB表达水平呈明显下降趋势(F=60.68,P<0.05),5-7d悬尾组NF-κB表达水平接近对照组,差异无统计学意义.NF-κB阳性产物主要见于实验大鼠肝细胞内,亦见于炎细胞及Kupffer细胞内,可分为胞质型、核型、核浆型等三个类型,单独或混合存在.结论:模模拟失重使大鼠肝脏组织中NF-κB表达发生明显变化,提示在失重环境中肝脏NF-κB的早期高表达和逐渐恢复过程与失重应激反应及失重耐受有密切关系.AIM： To investigate the expression of nuclear factor-kappa B （NF-κB） in liver tissus of rats under the condition of simulated weightlessness. METHODS： Eighty-four male adult Wistar rats weighing 280-310 g were randomly assigned to simulated weightlessness group and control group, each with 7 subgroups from 1 to 7 according time sequence in day unit. Tail-suspension was used to simulate the weightlessness condition. The expression of NF-κB p65 was de- tected by Western blotting analysis and PV-6001 immunohistochemistry respectively. RESULTS： The tail-suspended rats were upset at the beginning, and seemed adapted to the microgravity condition after 2 to 3 days. Tailsuspension significantly increased liver NF-κB expression in rats, as compared with the controls （F = 271.36, P 〈 0.01）, with peak expression on day I and 2, followed by a gradual decline to the normal level on day 5, 6 and 7 （F = 60.68, P 〈 0.05）. NF-κB expression stained as brown particles was mainly detected in rat hepatocytes, also in the infiltrated cells and Kupffer cells. There were three types of intracellular expression according to the location of positive NF-κB particles, i.e. cytoplasm, nucleus, and cytoplasm plus nucleus, existing alone or co-existing in rat liver. CONCLUSION： Simulated weightlessness, especially in the early stage, acts as a kind of stress to induce the activation of NF-κB in liver tissues of rats, suggesting that NF-κB plays an important role in the cascade reactions and adaptation to the weightlessness stress.

关 键 词：模拟失重 大鼠 肝脏 核因子ΚB 蛋白免 疫印迹法 免疫组化 

分 类 号：R85[医药卫生—航空、航天与航海医学]