盘状红斑狼疮皮损角质形成细胞的蛋白组学初步分析  

Preliminary Proteomics Analysis of Keratinocytes from Discoid Lupus Erythematosus

作　　者：方圣[1] 陈明春[1] 郭庆[1] 曾凡钦[1] 李伯友[1] 朱晓浚[1] 

机构地区：[1]中山大学附属第二医院皮肤科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2008年第6期749-752,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金(5001781)

摘　　要：【目的】建立盘状红斑狼疮皮损和正常皮肤角质形成细胞的双向电泳图谱,分析两者蛋白质的表达差异及特点。【方法】运用固相pH梯度双向凝胶电泳分离6例盘状红斑狼疮患者皮损及正常皮肤角质形成细胞总蛋白,考马斯亮蓝染色后用ImageMaster 2D图象软件比较分析,识别差异蛋白。【结果】盘状红斑狼疮皮损和正常对照电泳图谱平均蛋白点分别为1566±32和1682±38,平均匹配点数为1286±14和1314±21,匹配率为82.12%和78.12%。分辨差异蛋白点共22个,11个在皮损高表达,2个在皮损组低表达,有5个点仅在病例组表达,4个点仅在正常对照组表达。【结论】成功获得分辨率高且重复性较好的蛋白质组双向电泳图谱,识别重复表达的差异蛋白,为后续蛋白功能研究奠定基础。[ Objective ] To establish 2-dimensional electrophoresis profiles of the protein in keratinocytes from exanthemata and normal skin of discoid lupus erythematosus patients and analyze the difference in protein expression between the two groups. [Methods] The total proteins of keratinocytes were separated by immobilized pH gradient 2-dimensional gel electrophoresis （2-DE）. The differential protein expression was analyzed using ImageMaster 2D analysis software after staining. [Results] In the two groups, the average protein spots were 1 566 ± 32 and 1 682 ± 38, the average matching rates were 82.12% and 78.12%. Twenty-two differential protein spots were identified, 11 spots were highly expressed and 2 poorly expressed in the group of discoid lupus erythematosus; 5 protein spots were expressed only in the the group of discoid lupus erythematosus while 4 in the normal control group. [ Conclusion ] The 2-dimensional electrophoresis profiles with high resolution and reproducibility of keratinocytes are established.The study on these differential proteins expression will lay a strong foundation for further protein functional research.

关 键 词：盘状红斑狼疮 角质细胞 蛋白质组 

分 类 号：R75[医药卫生—皮肤病学与性病学]