内含肽介导PHB纯化人源抗菌肽LL-37体系的构建  

System of Intein-mediated PHB Purified Human Antimicrobial Peptide LL-37

作　　者：顾银霞[1] 周学章[1] 宋振威[1] 王玉炯[1] 

机构地区：[1]宁夏大学生命科学学院西部特色生物资源保护与利用教育部重点实验室,银川750021

出　　处：《中国生物工程杂志》2008年第11期72-76,共5页China Biotechnology

基　　金：国家"973"计划项目子课题项目(2006CB504401)资助

摘　　要：新颖的内含肽介导PHB纯化蛋白体系,是一种高效表达、自动切割、纯化方便、费用低廉的蛋白表达纯化体系,有利于蛋白规模化纯化。选用对原核细胞具有毒害作用的小肽——人源抗菌肽LL-37作为纯化对象,通过基因工程技术,构建内含肽介导PHB纯化人源抗菌肽LL-37体系,并利用该体系纯化LL-37。结果表明,构建的内含肽介导PHB纯化人源抗菌肽LL-37体系可高效表达LL-37融合蛋白,利用构建的纯化体系能对目的蛋白进行纯化。The new intein-mediated PHB purify protein system is a high expression, automatic cutting, for purification, low-cost protein purification system,it is conducive to large-scale protein purification. Choose human antibacterial peptide LL-37 as the purification objects, which is poison to prokaryotic cell. We construct inteinmediated PHB purified human antimicrobial peptide LL-37 system through genetic engineering technology and use this system to purify LL-37. The results show that this system can highly express LL-37 fusion protein and purifiy the product as same size with expectations.

关 键 词：蛋白表达聚 β-羟基丁酸酯(PHB) 内含肽 人源抗菌肽LL-37 

分 类 号：Q819[生物学—生物工程]