亚急性与慢性砷暴露人群尿砷和8-羟基脱氧鸟苷含量的比较  

Comparison of Urinary Arsenic and 8-hydroxy-2'-deoxyguanosine between Subacute and Chronic Arsenic-Exposed Subjects

作　　者：徐苑苑[1] 李昕[1] 王毅[1] 李冰[1] 郑全美[1] 王惠惠[1] 曲广[2] 孙贵范[1] 

机构地区：[1]中国医科大学公共卫生学院劳动卫生教研室,辽宁沈阳110001 [2]阜新市中心医院消化内科,辽宁阜新123000

出　　处：《环境与健康杂志》2008年第11期946-949,共4页Journal of Environment and Health

基　　金：国家自然科学基金重点资助项目(30530640)

摘　　要：目的比较亚急性与慢性砷暴露人群尿砷水平、砷形态分布和DNA氧化损伤的情况。方法选择2004年12月阜新市亚急性砷中毒事件发生时阜新市中心医院收治的65名男性患者(年龄为20～62岁,水砷含量达48.5 mg/L,连续暴露4～7 d)为亚急性砷暴露组,慢性高砷(男性72人,年龄为18～73岁,水砷浓度为0.24 mg/L,暴露6 a)、低砷暴露人群(男性40人,年龄为18～60岁,水砷浓度为0.02 mg/L,暴露10 a,无砷中毒相关临床症状)均选自内蒙古呼和浩特市周边砷暴露区。采集亚急性砷暴露人群确诊入院且未采取治疗措施时的尿样,采集慢性砷暴露人群即时尿样。采用超低温捕集-氢化物发生-原子吸收分光光度法测定尿中不同形态砷含量,用酶联免疫法分析尿8-羟基脱氧鸟苷(8-OHdG)水平。结果3种不同砷暴露人群尿中总砷水平和不同形态砷水平均随着砷暴露剂量的升高呈升高趋势:亚急性砷暴露组>慢性高砷暴露组>慢性低砷暴露组,且差异均有统计学意义(P<0.05)。各组尿中无机砷、一甲基砷和二甲基砷含量占总砷含量的构成比(iAs%,MMA%和DMA%)间比较,差异均有统计学意义(P<0.05)。iAs%和MMA%在亚急性砷暴露组中最高,在慢性低砷暴露组中最低,而DMA%在亚急性砷暴露组中最低,在慢性低砷暴露组中最高。亚急性砷暴露组、慢性高、低砷暴露组尿中8-OHdG水平分别为33.46(95%CI为28.73～38.96 ng/mg Cr)、2532(95%CI为21.10～30.16 ng/mg Cr)和9.52 ng/mgCr(95%CI为7.42～12.21 ng/mg Cr),3者间比较,差异均有统计学意义(P<0.05)。亚急性砷暴露人群和慢性高、低砷暴露人群尿中8-OHdG水平均与尿总砷水平呈正相关,Pearson相关系数分别为0.849(P<0.01),0.555(P<0.01)和0.733 (P<0.01)。尿中8-OHdG水平与砷甲基化模式相关性分析结果显示,亚急性砷暴露人群尿中8-OHdG水平与尿中各形态砷构成比间无相关关系(P>0.01);慢性高、低砷暴露人群尿中8-OHdG水平均与MMA%呈�Objective To compare urinary arsenic species and oxidative DNA lesions between subacute and chronic arsenicexposed subjects. Methods Sixty-five male patients（aged 20-62 years, concentration of arsenic in drinking water was 48.5 mg/L, time of arsenic exposure was 4-7 d） hospitalized in the central hospital of Fuxin during the accident of subacute arsenic poisoning in Fuxin city in December 2004 were selected as the subaeute arsenic-poisoning group, The chronic high-（72 males, aged 18-73 years, concentration of arsenic in drinking water was 0.24 mg/L, time of arsenic exposure was 6 a） and low-arsenic-exposed subjects （40 males, aged 18-60 years, concentration of arsenic in drinking water was 0.02 mg/L, time of arsenic exposure was 10 a, without clinical symptoms of arsenicosis） were selected from arsenic contaminated areas around Hohhot, Inner Mongolia. The urine samples of subaeute arsenic-poisoning subjects were collected immediately when they got into the hospital and before any treatment. The urine samples of chronic arsenic-exposed subjects were collected on site. The concentrations of urinary arsenic species were determined with cold trap hydride generation atomic absorption spectrometry. The level of urinary 8-hydroxy-2＇-deoxyguanosine （8-OHdG） was analyzed with the enzyme-linked immunosorbent assay kit. Results In the arsenic-exposed groups, the levels of urinary arsenic species and total arsenic showed increase trends as the dose of arsenic exposure increased： subaeute arsenicexposed group〉chronic high-arsenic-exposed group〉chronic low-arsenic-exposed group, and the differences among the 3 groups were significant（P〈0.05）. The differences in urinary iAs%, MMA% and DMA% among the 3 groups were also significant（P〈0.05）. iAs% and MMA% were the highest in the subacute arsenic-poisoning group, and lowest in the chronic low-arsenic-exposed group, DMA% were the lowest in the subacute arsenic-poisoning group, and the highest in the chronic low-arsenic-exposed group, The levels of

关 键 词：砷 甲基化 8-羟基脱氧鸟苷 

分 类 号：R994.6[医药卫生—毒理学]