石岐杂鸡胰岛素样生长因子-Ⅰ基因型与产蛋性状相关性研究  

Analysis on the correlation between insulin-like growth factor-Ⅰ genotypes and egg laying performance of Shiqiza yellow hens

作　　者：俞明月[1,2] 周震祥 顾彩菊 李震 蓝胜芝[1,2] 曹祥荣[1] 黄启忠 

机构地区：[1]南京师范大学生命科学学院,南京210097 [2]上海市农业遗传育种重点实验室,上海201106 [3]上海市农业科学学院畜牧兽医研究所,上海201106

出　　处：《上海农业学报》2008年第4期39-41,共3页Acta Agriculturae Shanghai

基　　金：上海市2004年度科技兴农攻关项目(沪农科攻字(2004)第5-5号)资助

摘　　要：采用PCR-RFLP技术对石岐杂黄鸡351个样本的胰岛素样生长因子-Ⅰ(IGF-Ⅰ)基因的5’端调控区进行遗传多态性研究,发现经PstⅠ酶切后出现3种基因型:AA、AB和BB,基因型分布符合哈代-温伯格定律。3种基因型与开产日龄(AFL)、50周龄产蛋量(EP50w)相关性分析结果显示,不同基因型开产日龄差异不显著;50周龄产蛋量,AA型最低,AA型与AB型有显著差异,AA型与BB型差异极显著,BB型与AB型差异不显著。试验结果表明等位基因B可能同IGF-Ⅰ基因表达调控有关,或同产蛋性状基因相连锁。试验为以IGF-Ⅰ基因型为分子标记进行辅助育种研究提供了基础。The 351 Shiqiza yellow hens from this institutes hennery were used as test materials and the genetic polymorphism of the 5＇region of their insulin-like growth factor-Ⅰ （IGF-Ⅰ ）was studied by means of PCR-RFLP technology. It was found that through Pst Ⅰ digestion,AA,AB and BB three gen- otypes appeared, and their distribution accorded with the Hardy-Weinberg law. The correlation analysis of the three genotypes with the age at first laying（AFL）and egg production at age 50 weeks （EP50w）showed that there was not a significant difference in the AFL among different IGF-Ⅰ genotypes, but there were significant differences in the EPS0w among different IGF-Ⅰ genotypes. The experimental result indicated that the allele B could be related to the IGF-Ⅰ gene expression or link with the egg laying performance-related genes.

关 键 词：鸡 IGF-Ⅰ基因 遗传多态性 PCR-RFLP产蛋性状 

分 类 号：S831[农业科学—畜牧学]